| 刘美清,兰英,蔡曼,等.产黏液分枝杆菌的生物学特征及快速药敏试验分析[J].安徽医药,2016,20(1):159-162. |
| 产黏液分枝杆菌的生物学特征及快速药敏试验分析 |
| Biological characteristics of mucus producing mycobacterium and fast susceptibility testing |
| 投稿时间：2015-07-06 |
| DOI： |
| 中文关键词: 非结核分枝杆菌 产黏液分枝杆菌 16S rRNA 菌种鉴定 聚合酶β亚单位 |
| 英文关键词: non-tuberculous mycobacteria mucus producing mycobacterium 16S rRNA strain identification rpoB |
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| 中文摘要: |
| 目的 了解产黏液分枝杆菌的生物学特征,并进行菌种鉴定和药敏分析,为临床准确诊断和治疗提供科学依据。方法 从患者血需养培养瓶中分离出的可疑菌株,经生长时间、快速革兰染色及萋-尼氏法抗酸染色初筛后,基于16S rRNA基因及rpoB基因序列分析检测菌株种类,并进行相应的快速药物敏感性检测。结果 最终鉴定为非结核分枝杆菌中的产黏液分枝杆菌,属于快速生长分枝杆菌类；K-B法体外药敏试验显示,抑菌环直径30 mm以上从大到小依次为:克拉霉素、阿米卡星、头孢克罗、头孢噻肟、氨苄西林、哌拉西林、阿莫西林﹣克拉维酸、亚胺培南、奈替米星、阿奇霉素。结论 产黏液分枝杆菌有致病性,可引起菌血症,临床应根据患者自身情况,尽早拔除导管,需进行抗感染治疗时依据药敏结果合理用药,防止院内感染的发生。 |
| 英文摘要: |
| Objective To explore the biological characteristics of mucus producing mycobacterium and carry out strain identification as well as susceptibility analysis to provide a scientific basis for accurate clinical diagnosis and treatment. Methods Suspected strains, isolated from the blood aerobic culture flasks of patients, were screened by the growth time, rapid Gram stain and Ziehl - Nigeria 's acid-fast staining. The strains were detected on 16S rRNA gene and rpoB gene sequence analysis and the corresponding rapid drug susceptibility testing.Results Suspected strains were identified as mucus producing mycobacterium of non-tuberculous mycobacteria, a rapidly growing mycobacteria class. KB vitro susceptibility testing showed that drugs with zone diameters above 30mm in descending order were clarithromycin, amikacin, cefaclor, cefotaxime, ampicillin, piperacillin, amoxicillin/clavulanic acid, imipenem, netilmicin, and azithromycin. Conclusions Mucus producing mycobacterium is pathogenic and can cause bacteremia. To prevent nosocomial infection, we should remove urethral catheter as early as possible according to the patient's own condition and use rational drug based on susceptibility results. |
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