| 许广华,王虎,李小伟,等.芪精愈消胶囊薄层鉴别及含量测定研究[J].安徽医药,2016,20(4):660-663. |
| 芪精愈消胶囊薄层鉴别及含量测定研究 |
| Study on TLC identification and content determination of qijing yuxiao capsules |
| 投稿时间：2015-09-30 |
| DOI： |
| 中文关键词: 芪精愈消胶囊 五味子 黄芪 葛根 葛根素 |
| 英文关键词: qijing yuxiao capsules schisandra chinensis astragalus membranaceus root of kudzu vine puerarin |
| 基金项目: |
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| 摘要点击次数: 2690 |
| 全文下载次数: 174 |
| 中文摘要: |
| 目的 制定芪精愈消胶囊的质量标准。方法 采用TLC法鉴别处方中的黄芪、五味子；采用HPLC法测定葛根中葛根素的含量,Agilent C18色谱柱(4.6 mm×250 mm,5 μm),流动相为甲醇—0.4%磷酸溶液(25∶75),检测波长250 nm。结果 黄芪、五味子薄层鉴别斑点清晰,阴性无干扰,可作为定性鉴别；含量测定中葛根素在0.169 4～0.846 8 μg范围内,与峰面积线性关系良好,回归方程为A=4375 947C+1 708(r=0.999 9),回收率平均为99.36%,RSD=0.98%(n=5)。 结论 该方法操作简单,专属性和重现性良好,可作为该制剂的质量控制方法。 |
| 英文摘要: |
| Objective To establish the quality standard of qijing yuxiao capsules. Methods The TLC method was used to identify schisandra chinensis,and astragalus membranaceus.HPLC method was used to determine the contents of puerarin. The optimal conditions of the separation and detection were achieved on a Agilent C18 column with a mobile phase consisting of methanol-0.4% phosphoric acid solution(25∶75) at the detective wavelength of 250 nm. Results TLC spots of radix astragalus and schisandra chinensis were clear and negative without interference, which can be used as qualitative method. The contents of puerarin had a good linear relation with peak area(r=0.999 9), when ranging from 0.169 4～0.846 8 μg. and equation was A=4375 947C+1 708.The average recovery of puerarin was 99.36% and RSD was 0.98%(n=5).Conclusion The method is simple,with good specificity and reproducibility,and can be used as the quality control for this preparation. |
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