| 汪琛媛,李瑛,汪永忠.痺苓祛痛颗粒的UPLC指纹图谱研究[J].安徽医药,2016,20(5):863-865. |
| 痺苓祛痛颗粒的UPLC指纹图谱研究 |
| UPLC fingerprint of bilingqutong granule |
| 投稿时间：2015-12-07 |
| DOI： |
| 中文关键词: 色谱法,高压液相 质量控制 痺苓祛痛颗粒 |
| 英文关键词: Chromatography,High Pressure Liquid Quality Control bilingqutong granul |
| 基金项目: |
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| 摘要点击次数: 2629 |
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| 中文摘要: |
| 目的 建立超高效液相色谱法(UPLC)研究痺苓祛痛颗粒指纹图谱,为其质量控制提供依据。 方法 色谱柱为Acquity BEH C18(2.1 mm×100 mm,1.7 μm),流动相为乙腈-0.05%磷酸水溶液,梯度洗脱；流速为0.25 mL·min-1；检测波长为254 nm。结果 建立了10批痺苓祛痛颗粒的共有图谱,相似度均大于0.97,确定了13个共有峰。结论 所建立的痺苓祛痛颗粒的指纹图谱特征性和专属性强,该方法可用于痺苓祛痛颗粒的质量控制。 |
| 英文摘要: |
| Objective To establish an ultra high performance liquid chromatographic (UPLC) fingerprint for the quality control of bilingqutong granule,to provide a comprehensive evaluation for its quality control.Methods UPLC separation was performed on an Acquity BEH C18(2.1 mm×100 mm,1.7 μm) by gradient elution with 0.05 % phosphoric acid-acetonitrile as mobile phase.The flow rate was 0.25 mL·min-1 and the detection wavelength was set at 254 nm.Results Thirteen characteristic common peaks of chemical constituents were identified and 10 batches of bilingqutong granule were analyzed.The quanlity of samples was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A version).The similarities of 10 batches of bilingqutong granule were all over 0.97,and 13 characteristic peaks of UPLC fingerprint for bilingqutong granule were established.Conclusions The established UPLC fingerprint of bilingqutong granule is convenient and highly specific,which could be used to identify and evaluate the quality of bilingqutong granule. |
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