| 沈秀珍,王凌,方毅华.银杏总黄酮通过调节自噬减轻大鼠心肌缺血再灌注损伤的研究[J].安徽医药,2016,20(6):1065-1067. |
| 银杏总黄酮通过调节自噬减轻大鼠心肌缺血再灌注损伤的研究 |
| Yinxing leaf total flavonoid mitigates myocardial ischemia and reperfusion injury via regulating autophagy |
| 投稿时间：2016-02-23 |
| DOI： |
| 中文关键词: 再灌注损伤 银杏素 自噬 大鼠,Sprague-dawley |
| 英文关键词: Reperfusion Injury Ginkgetin Autophagy Rats,Sprague-dawley |
| 基金项目: |
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| 摘要点击次数: 2530 |
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| 中文摘要: |
| 目的 探讨银杏总黄酮是否可以通过调节自噬减轻心肌缺血再灌注(I/R)损伤以及可能的机制。方法 采用大鼠心肌I/R模型(缺血30 min,再灌注4 h),雄性SD大鼠30只随机分为三组:假手术组(SO组,n=10)、缺血再灌注组(I/R组,n=10)和银杏总黄酮治疗组(YLTF+I/R组,n=10,100 mg·kg-1,造模前灌胃7 d)。检测心肌损伤标记物肌酸激酶(CK)、乳酸脱氢酶(LDH)和肌钙蛋白I(cTnI)；检测氧化应激水平,即丙二醛(MDA)和超氧化物歧化酶(SOD)水平；检测自噬蛋白beclin-1和LC3水平。结果 与SO组比较,I/R可诱导心肌损伤标记物(CK、LDH和cTnI)表达升高,氧化应激水平的增加(MDA表达增加,SOD表达降低)和自噬的过度激活(LC3-Ⅱ/Ⅰ比值增加,beclin-1表达增加)；与I/R比较,银杏总黄酮可显著的降低心肌损伤标记物(CK、LDH和cTnI)的表达、氧化应激水平(MDA表达降低,SOD表达增加)和自噬的过度激活(LC3-Ⅱ/Ⅰ比值降低,beclin-1表达减低)。结论 银杏总黄酮可以通过抑制过度自噬减轻心肌I/R损伤,这可能与其抑制氧化应激作用有关。 |
| 英文摘要: |
| Objective To investigate whether Yinxing leaf total flavonoid could mitigate myocardial ischemia and reperfusion(I/R) injury and the possible mechanism.Methods Rats I/R model (ischemia 30 min,then reperfusion 4 h) was used.30 rats were divided into three groups:sham-operation group (SO,n=10),ischemia and reperfusion group (I/R,n=10) and Yinxing leaf total flavonoid treatment group (YLTF+I/R,n=10,0 mg·kg-1).Cardiac injury markers (serum creatine(CK),lactate dehydrogenase(LDH),and cTnI),oxidative stress (malondialdehyde (MDA) and superoxide dismutase (SOD)) and autophagy markers (beclin-1 and LC3) were detected.Results Compared with SO group,levels of cardiac injury markers (CK,LDH and cTnI) and oxidative stress (MDA and SOD) significantly increased; autophagy was excessively activated in I/R group.Compared with I/R group,Yinxing leaf total flavonoid significantly decreased cardiac injury markers'(CK,LDH and cTnI) expression and oxidative stress (MDA expression increased and SOD expression decreased),and suppressed excessive activation of autophagy both beclin-1 level and LC3-Ⅱ/Ⅰ ratio decreased).Conclusions Yinxing leaf total flavonoid could mitigate I/R injury via inhibiting excessive activation of autophagy.It's possible that Yinxing leaf total flavonoid inhibited excessive autophagy via suppressing oxidative stress. |
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