| 张颖,宋志钊,刘元.高效液相色谱法测定“天竺痫康宁胶囊”中胆红素的含量[J].安徽医药,2016,20(6):1087-1089. |
| 高效液相色谱法测定“天竺痫康宁胶囊”中胆红素的含量 |
| Determination of bilirubin in Tianzhu Xiankangning capsules by HPLC |
| 投稿时间：2015-12-01 |
| DOI： |
| 中文关键词: 胆红素/分析 色谱法,高压液相 天竺黄 胆南星 中草药 |
| 英文关键词: Bilirubin/analysis Chromatography,high pressure liquid Concretio silicea bambusae Arisaema cum bile Drugs,chinese herbal |
| 基金项目:广西壮族自治区卫生厅中医药科技专项(GZZJ14-11);南宁市科学研究与技术开发计划(20143135) |
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| 中文摘要: |
| 目的 建立天竺痫康宁胶囊胆红素含量的高效液相色谱(HPLC)测定方法。方法 采用Intersil-C18色谱柱,流动相为甲醇-三氯甲烷-1%磷酸(80∶14∶6),流速1.0 mL·min-1,检测波长为450 nm,柱温为室温,进样量为10 μL。结果 在该条件下,浓度为2.26～45.2 mg·L-1范围内,胆红素峰面积与其浓度呈良好线性关系(r=0.999 6),平均回收率为97.4%,RSD为2.02%(n=6)。结论 该方法灵敏、简便、准确、重复性好,可作为天竺痫康宁胶囊质量标准的控制指标之一。 |
| 英文摘要: |
| Objective To establish an HPLC method for the determination of bilirubin in Tianzhu Xiankangning capsules.Methods The Intersil-C18 column was used,and the mobile phase consisted of Methanol-trichloromethan-1% phosphoric acid (80∶14∶6) with the flow rate at 1.0 mL·min-1.The detection wave length was 450 nm and the sample size was 10 μL.Results Linearity of bilirubin was good around 2.26～45.2 mg·L-1,r=0.999 6.The average recovery was 97.4% with RSD less than 2.02% (n=6).Conclusions The method is sensitive,simple,accurate and repeatable,which can be applied to the determination of bilirubin in Tianzhu Xiankangning capsules. |
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