| 董巍,郝修洁,王超众,等.高效液相色谱法测定对叶百部根蜜炙后对叶百部碱的含量[J].安徽医药,2016,20(8):1483-1485. |
| 高效液相色谱法测定对叶百部根蜜炙后对叶百部碱的含量 |
| Changes of tuberostemonine in root of Stemona tuberosa before and after stir-frying with honey by HPLC |
| 投稿时间：2016-05-01 |
| DOI： |
| 中文关键词: 百部/化学 蜜炙 色谱法,高压液相 |
| 英文关键词: Stemona japonica/chemistry Honey processing Chromatography,high pressure liquid |
| 基金项目:黑龙江省教育厅科学技术研究项目(12541925) 作者简介:董巍,女,博士,研究方向:中药药效物质基础研究,E-mail:pingguoweiweiwei@126.com高效液相色谱法测定对叶百部根蜜炙后对叶百部碱的含量董巍1,郝修洁2,王超众2,郭丽娜1,潘虹1,林宇1,李晓明1 (1.齐齐哈尔医学院药学院,黑龙江齐齐哈尔 161006；2.齐齐哈尔市食品药品检验检测中心,黑龙江齐齐哈尔 161006)高效液相色谱法测定对叶百部根蜜炙前后对叶百部碱的含量变化 董巍,郝修洁,王超众,等 Changes of tuberostemonine in Root of Stemona tuberosa before and after stir-frying with honey by HPLC DONG Wei1,HAO Xiujie2,WANG Chaozhong2,et al |
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| 中文摘要: |
| 目的 采用高效液相色谱法(HPLC)测定对叶百部根蜜炙前后对叶百部碱的含量。方法 采用反相高效液相色谱法(RP-HPLC),使用迪马Diamonsil C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.2%氨水流动相,流速1.0 mL·min-1,柱温35 ℃,检测波长254 nm,进样量20 μL。结果 对叶百部碱线性范围为31.25~500.0 mg·L-1,r= 0.999 5,平均加样回收率(n=9)为99.88%,RSD为0.26%。不同产地对叶百部根中对叶百部碱含量差异较大,其中河北对叶百部根中对叶百部碱含量较高为1514.80 μg·g-1,广西产对叶百部碱含量较低为197.40 μg·g-1。蜜炙后河北、广西产对叶百部根中对叶百部碱含量分别为919.60、129.76 μg·g-1。结论 该文建立的含量测定方法符合要求。对叶百部根经蜜炙后对叶百部碱的含量下降,表明蜜炙对叶百部根中生物碱类成分的含量产生了一定影响。 |
| 英文摘要: |
| Objective To establish an HPLC method for the determination of tuberostemonine in root of Stemona tuberosa Lour.during stir-frying with honey.Methods HPLC and Diamonsil-C18 column (250 mm×4.6 mm,5 μm) were adopted,with acetonitrile-0.2% ammonia as the mobile phase at the flow rate of 1.0 mL·min-1.The column temperature was set at 35 ℃,and the UV detection wavelength was 254 nm.Sample quantity was 20 μL.Results Tuberostemonine showed a good linear relationship within the range of 31.25~500.0 mg·L-1(r=0.999 5),with the average recovery of 99.88% (n=9),RSD being 0.26%.Tuberostemonine contents of Stemona tuberosa Lour.in different areas had great difference.The content of Stemona tuberosa Lour.purchased from Hebei was the highest,1514.80 μg·g-1,and the lowest was purchased from Guangxi,197.40 μg·g-1.After stir-frying with honey,the content of tuberostemonine were decreased to 919.60,9.76 μg·g-1,respectively.Conclusions The method was simple and reliable,which met the requirements.The content of tuberostemonine is decreased by stir-frying with honey,indicating that honey-processed approach had an effect on the content of alkaloids in Stemona tuberosa Lour. |
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