| 谢玉惠,龙兴浩.离子色谱法同时测定附子理中丸中9种无机阴离子含量[J].安徽医药,2016,20(8):1490-1493. |
| 离子色谱法同时测定附子理中丸中9种无机阴离子含量 |
| Determination of 9 inorganic anions in Fuzi Lizhong Pills by ion chromatography |
| 投稿时间：2016-05-20 |
| DOI： |
| 中文关键词: 附子理中汤 阴离子 硝酸盐类 亚硝酸盐类 氯化物 溴化物 氟化物 色谱法,离子交换 |
| 英文关键词: Fuzi lizhong decoction Anions Nitrates Nitrites Chlorides Bromides s Fluorides Chromatography,ion exchange |
| 基金项目: |
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| 摘要点击次数: 2489 |
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| 中文摘要: |
| 目的 建立同时测定附子理中丸中9种无机阴离子(氟离子、甲酸根离子、氯离子、亚硝酸根离子、溴离子、硝酸根离子、硫酸根离子、草酸根离子和磷酸根离子)含量的离子色谱法。方法 采用Ion Pac AS11-HC阴离子交换分析色谱柱,ASRS 300(250 mm×4 mm)阴离子抑制型电导检测器检测,7～40 mmol·L-1氢氧化钾溶液梯度淋洗20 min,平衡5 min,流速为1.0 mL·min-1,柱温为35 ℃,进样量为20 μL。结果 9种无机阴离子达到良好分离,0.1～10 mg·L-1的线性关系良好,相关系数为0.999 0～0.999 8,精密度的RSD为0.5%～1.7%,重复性的RSD为0.8%～2.7%,稳定性的RSD为1.1%～2.9%,平均加样回收率为89.18%～106.81%间,RSD为0.51%～2.73%(n=6),检测限为0.004～0.023 mg·L-1,定量限为0.015～0.018 mg·L-1。结论 该方法快速简单,选择性好,灵敏度高,适用于附子理中丸中9种无机阴离子的含量测定。 |
| 英文摘要: |
| Objective To establish the method for simultaneous determination of nine inorganic anions (fluoride,formate,chloride,nitrite,bromide,nitrate,sulfate,radical,oxalate and phosphate) in Fuzi Lizhong Pills by ion chromatography.Methods Nine inorganic anions were determined by anion exchange column Ion Pac AS11-HC (250 mm×4 mm),and eluted by gradient hydroxide potassium in 7～40 mmol·L-1.The test took 20 min to determine targets and took 5 min to balance column.And the test was identified by suppressed conductivity detector ASRS 300 (4 mm) in the rate of 1.0 mL·min-1,and determined at the column temperature of 35 ℃ and the sampling volume of 20 μL.Results Good resolution can be achieved among the above mentioned nine inorganic anions.The nine inorganic anions showed a good linear relationship in the range of 0.1～10 mg·L-1,and their linear correlations over the investigated concentration were in the range of 0.999 0～0.999 8,while RSDs of precision were 0.5%～1.7%,RSDs of repeatability 0.8%～2.7%,and RSDs of stability 1.1%～2.9%.The average recoveries were 89.18%～106.81% with relative standard deviation between 0.51% and 2.73%,respectively.Conclusions The ion chromatography method is fast,simple,well selective,and well sensitive for the assay and quality control of nine inorganic anions in Fuzi Lizhong Pills. |
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