| 刘园,张华峰,宋青,等.高效液相色谱法测定甲癣涂剂中水杨酸的含量[J].安徽医药,2016,20(11):2041-2042. |
| 高效液相色谱法测定甲癣涂剂中水杨酸的含量 |
| Determination of salicylic acid in paint for onychomycosis by HPLC |
| 投稿时间：2016-08-04 |
| DOI： |
| 中文关键词: 高效液相色谱法 甲癣涂剂 水杨酸 |
| 英文关键词: HPLC Paint for onychomycosis Salicylic acid |
| 基金项目: |
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| 摘要点击次数: 2368 |
| 全文下载次数: 2 |
| 中文摘要: |
| 目的 建立甲癣涂剂中水杨酸的含量测定方法。方法 色谱柱:Venusil XBP(L)C18柱(4.6 mm×100 mm,5 μm)为固定相,甲醇∶水(H3PO4调pH=3.0)=45∶55为流动相,检测波长303 nm,流速1.0 mL·min-1,柱温:20 ℃。进样量 20 μL。结果 水杨酸回归方程为:A=5.14×104C-1.06×104(r=0.999 9)；线性范围为:4.98~159.36 mg·L-1。平均回收率分别为98.0%、100.8%、100.5%。结论 该方法操作方便,结果准确可靠,可用于甲癣涂剂中水杨酸的含量测定。 |
| 英文摘要: |
| Objective To establish a method for determination of salicylic acid in paint for onychomycosis by HPLC.Methods The chromatographic analysis was carried out on a Venusil XBP-C18(L)column(4.6 mm×100 mm,5 μm)with methanol and water solution(the pH value was adjusted to 3.0 by phosphoric acid)as the mobile phase at a flow rate of 1.0 mL·min-1.The column temperature was 20℃ and sample size was 20 μL.The detection wavelength was 303 nm.Results Linear regression equation of salicylic acid was A=5.14×104C-1.06×104(r=0.999 9),and it was obtained in the range of 4.98~159.36 mg·L-1.The average recoveries were 98.0%,100.8%,and 100.5%.Conclusions The HPLC method shows a good separation,reproducibility and accuracy,which can be used to determine salicylic acid. |
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