| 韩福新,张蕊,马善波,等.微小RNA-29c在胶质瘤中的表达及其对细胞分裂周期蛋白42的调控作用和对细胞增殖的影响[J].安徽医药,2016,20(11):2067-2070. |
| 微小RNA-29c在胶质瘤中的表达及其对细胞分裂周期蛋白42的调控作用和对细胞增殖的影响 |
| Expression of microRNA-29c in gliomas and its effect on cell division cycle protein 42 and cell proliferation |
| 投稿时间：2016-04-13 |
| DOI： |
| 中文关键词: 胶质瘤 微小RNA-29c 细胞分流周期蛋白42 细胞增殖 |
| 英文关键词: Glioma MiRNA-29c Cell division cycle 42 Proliferation |
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| 摘要点击次数: 2212 |
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| 中文摘要: |
| 目的 探究微小RNA-29c(miRNA-29c)在胶质瘤中的表达水平以及表达水平对胶质瘤增殖的影响。方法 选取手术切除的胶质瘤组织标本80例,根据世界卫生组织肿瘤分类分级标准,将组织标本进行分级,同时收集非胶质瘤组织标本25例作为对照组。将上述收集的组织标本制作为微组织阵列。同时切取5 μm×5 μm组织切片留用蛋白细胞分裂周期蛋白42(CDC42)免疫组化检测以及miRNA-29c原位杂交检测。结果 非胶质瘤组织标本miRNA-29c表达水平明显高于胶质瘤组织标本表达,miRNA-29c表达水平随着肿瘤分化级别的提升而降低；非胶质瘤组标本CDC42表达水平明显低与胶质瘤组,且随着胶质瘤分化级别提高CDC42水平明显增加；miRNA-29c靶mRNA生物信息学预测结果提示人CDC42mRNA是miRNA-29c潜在的靶mRNA；人胶质瘤细胞U87MG经过miRNA-29c mimics 转染48 h后,转染组细胞miRNA-29c表达水平明显高于空白对照组以及Scr转染对照组,通过转染方式可将miRNA-29c mimics成功转入人胶质瘤细胞U87MG,并且能够成功表达；miRNA-29c mimics转染组CDC42表达明显低于两对照组,在人胶质瘤细胞U87MG中miRNA-29c可以降解CDC42 mRNA的表达,从而降低CDC42蛋白的表达水平；miRNA-29c mimics转染组人胶质瘤细胞增殖活性在48、72、96 h明显低于空白对照组与Scr转染对照组,miRNA-29c可以有效的抑制人胶质瘤细胞U87MG增殖活性。结论 miRNA-29c是人胶质瘤的有效抑瘤miRNA之一,miRNA-29c表达水平可作为临床上判断胶质瘤分化分级的参考依据,miRNA-29c表达水平的降低减少了对其下游基因CDC42的抑制作用,引起CDC42表达的异常增加,导致肿瘤细胞的异常增殖。研究结果显示miRNA-29c在胶质瘤的发生发展过程中起着重要的调控作用。 |
| 英文摘要: |
| Objective To explore the expression of miRNA-29c in gliomas and its effecton the proliferation of glioma cells.Methods Eighty samples of surgical resection of glioma were collected in the Department of Neurosurgery in The First Affiliated Hospital of The Fourth Military Medical University,thenglioma tissue specimens were classified according to WHO Tumor Classification Standards and 25 specimens of non glioma tissues were collected as the control group.Tissue samples were fabricated as micro arrays.Simultaneous removal of 5 μm×5 μm tissue section was retained cell protein division cycle protein 42(CDC42)immunohis to chemical detection and in situ miRNA-29c hybridization detection.Results The expression level of miRNA-29c in non glioma tissue specimen was significantly higher than that in glioma tissue specimen,which decreased with the increase of tumor differentiation level.The expression of CDC42 in non glioma group wassignificantly lower than that in glioma group,which increased significantly with the increase of tumor differentiation level.miRNA-29c target mRNA bioinformatics prediction results suggested that human CDC42 mRNA was the potential target mRNA of miRNA-29c.Forty-eight hours after human glioma cells U87MG were transfected by miRNA-29c mimics,the expression level of miRNA-29c transfected cells was significantly higher than the control group and Scr transfection group.miRNA-29c mimics could be successfully transfected into human glioma cells U87MG,and couldbe successfully expressed;the expression of CDC42 in miRNA-29c mimics transfection group was significantly lower than the two control groups.In human glioma cells U87MG miRNA-29c could degrade CDC42 mRNA expression,thereby reducing the expression level of CDC42 protein.In miRNA-29c mimics transfected group the proliferation activitiesof humanglioma cell in 48 hours,72 hours and 96 hours were significantly lower than those of blank control group and Scrtransfection control group.miRNA-29c could effectively inhibit the proliferation activity of human glioma U87MG cells.Conclusions miRNA-29c can effectively inhibitshuman glioma,the expression of which can be used as a clinical reference for the differentiation of glioma.The decreased expression of miRNA-29c reduces its inhibition of CDC42,which results in the abnormal increase in CDC42 and the abnormal proliferation of tumor cells.The results indicate that miRNA-29c plays an important role in the occurrence and development of gliomas. |
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