| 周畅,胡婷婷,王志刚,等.虎杖苷通过降血脂抗ApoE -/-小鼠动脉粥样硬化的作用[J].安徽医药,2016,20(12):2226-2229. |
| 虎杖苷通过降血脂抗ApoE -/-小鼠动脉粥样硬化的作用 |
| Polydatin protects against atherosclerosis via reducing the level of oxidized low-density lipoprotein in ApoE deficient mice |
| 投稿时间：2016-06-23 |
| DOI： |
| 中文关键词: 虎杖苷 动脉粥样硬化 高密度脂蛋白 低密度脂蛋白 总胆固醇 |
| 英文关键词: Polydatin Atherosclerosis High density lipoprotein Low density lipoprotein Total cholesterol |
| 基金项目:江苏省中医药局科技项目(YB2015049)虎杖苷通过降血脂抗ApoE -/-小鼠动脉粥样硬化的作用周畅1,胡婷婷1,王志刚2,3,孙晓艳2,3,蔡雪婷2,3,庞中化2,3,苏克雷2,3,曹鹏2,3,刘文1 (1.东南大学附属中大医院,江苏 南京 210009；2.南京中医药大学附属中西医结合医院,江苏 南京 210028；3.江苏省中医药研究院,江苏 南京 210028)虎杖苷通过降血脂抗ApoE -/-小鼠动脉粥样硬化的作用周畅,胡婷婷,王志刚,等 |
| 作者 | 单位 | | 周畅 | 东南大学附属中大医院,江苏 南京 210009 | | 胡婷婷 | 东南大学附属中大医院,江苏 南京 210009 | | 王志刚 | 南京中医药大学附属中西医结合医院,江苏 南京 210028
江苏省中医药研究院,江苏 南京 210028 | | 孙晓艳 | 南京中医药大学附属中西医结合医院,江苏 南京 210028
江苏省中医药研究院,江苏 南京 210028 | | 蔡雪婷 | 南京中医药大学附属中西医结合医院,江苏 南京 210028
江苏省中医药研究院,江苏 南京 210028 | | 庞中化 | 南京中医药大学附属中西医结合医院,江苏 南京 210028
江苏省中医药研究院,江苏 南京 210028 | | 苏克雷 | 南京中医药大学附属中西医结合医院,江苏 南京 210028
江苏省中医药研究院,江苏 南京 210028 | | 曹鹏 | 南京中医药大学附属中西医结合医院,江苏 南京 210028
江苏省中医药研究院,江苏 南京 210028 | | 刘文 | 东南大学附属中大医院,江苏 南京 210009 |
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| 中文摘要: |
| 目的 研究虎杖苷在ApoE-/-小鼠动脉粥样硬化治疗中的作用及其可能的机制。方法 采用高脂饲料连续喂养12周ApoE-/-小鼠,建立动脉粥样硬化动物模型,将已经形成动脉粥样硬化ApoE-/-小鼠分为模型组,虎杖苷组,阳性药(辛伐他汀)组,每组8只,阳性药(辛伐他汀)以0.05 g·kg-1·d-1灌胃,虎杖苷0.2 g·kg-1·d-1灌胃,正常对照及模型组分别给予同体积的蒸馏水,连续灌胃28 d后,取血和冠状动脉,采用HE染色对冠状动脉的形态学改变进行观察；检测血清中高密度脂蛋白,氧化型的低密度脂蛋白,总胆固醇的含量。结果 病理切片HE染色显示,虎杖苷组小鼠的血管组织形态较模型组有较明显的改善,和阳性药组结果类似,显示出虎杖苷对动脉粥样硬化具有较好的的治疗效果；模型组血液中氧化型低密度脂蛋白的含量约为正常对照的5.3倍,虎杖苷组血液中的氧化型低密度脂蛋白的含量约为正常对照的3倍,是模型组含量的56.6%,与模型组比较,虎杖苷可以显著的降低血清中氧化型低密度脂蛋白的水平(q=7.884,P<0.05)。 结论 虎杖苷可降低血脂,减少脂质的沉积,改善动脉粥样硬化血管组织的形态,具备治疗动脉粥样硬化的潜力。 |
| 英文摘要: |
| Objective To determine whether polydatin could protect against atherosclerosis in ApoE deficient mice and to further reveal the potential mechanism.Methods Male ApoE-/- deficient mice(8 weeks old) were obtained and were given high-fat diet,while the normal control group was given normal diet.These high-fat diet mice were assigned into three groups:model group,polydatin group 0.2 g·kg-1·d-1,and positive drug group(Simvastatin,0.05 g·kg-1·d-1).The normal control group and model group were given the same volume of distilled water.All mice were administered orally,which continued for 28 days.Then,all mice were killed;serum and coronary arteries were saved.HE staining of coronary arteries was established.Serum high density lipoprotein,oxidized low density lipoprotein,and total cholesterol were measured.Results By HE staining,the results showed that polydatin had an obvious improvement in vascular tissue morphology in comparison with the model group,and positive drug group showed similar results,i.e,polydatin on atherosclerosis had good therapeutic effect.Oxidized low density lipoprotein in the blood of model group was about 5.3 times that of normal controls.Oxidized low density lipoprotein in the blood of polydatin group was about three times that of the normal controls,and 56.6% of the content of model group.Compared with the model group,polydatin significantly(q=7.884,P<0.05) reduced the levels of serum oxidized low density lipoprotein.Conclusions Polydatin can reduce blood fat,decrease lipid deposition,and improve the morphology of atherosclerotic vascular tissue in the treatment of atherosclerosis. |
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