文章摘要
李尤玲,杨靖,李健,等.没食子鞣酸对日本血吸虫感染小鼠模型肝病理改变的影响及作用机制研究[J].安徽医药,2017,21(4):615-617.
没食子鞣酸对日本血吸虫感染小鼠模型肝病理改变的影响及作用机制研究
Anti-fibrotic effect and mechanism of Gallic tannin on the hepaticfibrosis mouse model infected with Schistosoma japonicum
投稿时间:2016-08-18  
DOI:
中文关键词: 没食子鞣酸  日本血吸虫  BABL/c小鼠  肝脏  组织病理学
英文关键词: 
基金项目:湖北省卫生计生血防专项(WJ2015XB032);十堰市科技局市级科学技术研究与开发专项基金项目(16K70);湖北医药学院研究生启动金资助计划项目(2015QDJZR08)
作者单位E-mail
李尤玲 湖北医药学院附属人民医院中西结合科,湖北 十堰 442000  
杨靖 湖北医药学院附属人民医院感染性疾病科,湖北 十堰 442000
湖北医药学院基础医学院病原学实验室,湖北 十堰 442000 
 
李健 湖北医药学院附属人民医院感染性疾病科,湖北 十堰 442000
湖北医药学院基础医学院病原学实验室,湖北 十堰 442000 
 
李刚 湖北医药学院附属人民医院感染性疾病科,湖北 十堰 442000 hr168@163.com 
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中文摘要:
      目的 在建立血吸虫肝纤维化小鼠模型基础上,研究没食子鞣酸对血吸虫感染所致肝纤维化的影响及作用机制。方法 利用日本血吸虫尾蚴感染BALB/c小鼠建立感染模型,将50只小鼠随机分为没食子鞣酸高、中、低浓度组、正常对照组和模型组等5组,每组10只。血吸虫感染4周后,各组分别给予相应的药物干预,模型组、正常对照组仅给予生理盐水,不予药物干预。没食子鞣酸连续灌胃4周后,观察小鼠的生活状况并记录小鼠体质量。实验开始8周后处死小鼠取标本做免疫组化检测结缔组织生长因子(CTGF)的表达;HE染色、Masson染色观察小鼠肝脏病理学变化。结果 与造模组小鼠相比,3个没食子鞣酸干预组肝肉芽肿面积较小,纤维化程度较轻,肝组织CTGF蛋白表达水平较低,均差异有统计学意义(P<0.05)。结论 没食子鞣酸可明显减轻日本血吸虫对小鼠感染模型肝病理学损伤,缓解肝肉芽肿所致肝纤维化程度。
英文摘要:
      Objective To investigate the anti-fibrotic effect of Gallic tannin in a schistosomiasis-induced hepatic fibrosis mouse model.Methods Fifty BALB/c mice were randomly assigned into Gallic tannin high concentration group,middle concentration group,low concentration group,model group and normal group.The first four groups were infected by Schistosoma japonicum cercaria.After 4 weeks infection,the groups were given appropriate medication,control group received saline.Meanwhile,each group had intragastric administration one time every day for 4 weeks.Then we observed living conditions,and recorded the mice body weight.Specimens were detected by immunohistochemistry the expression of CTGF,HE staining,MASSON staining pathological changes in the liver of mice.Results The degree of fibrosis,the expression of CTGF protein in the three Gallic tannin groups were less than model group,the differences were statistically significant (P<0.05).Conclusions Gallic tannic can significantly reduce the liver pathology injury induced by Schistosoma japonicum cercaria,and relieve the degree of liver fibrosis.
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