文章摘要
王奕,陈雪辉,陈红兵.2型糖尿病发病进程中血清微小核糖核酸-103表达的临床意义[J].安徽医药,2017,21(10):1840-1843.
2型糖尿病发病进程中血清微小核糖核酸-103表达的临床意义
The clinical significance of expression level of serum microRNA-103 in the progression of type 2 diabetes mellitusWANG Yi1,CHEN Xuehui2,CHEN Hongbing1 (1.Department of Clinical Laboratory, The Affiliated Children′s Hospital of Nanjing Medical University, Nanjing,Jiangsu 210008,China;2.Department of Clinical Laboratory,Nanjing Hospital of Chinese Medicine, Nanjing,Jiangsu 210001,China)Abstract:Objective
投稿时间:2016-10-16  
DOI:
中文关键词: 糖调节受损  2型糖尿病  糖尿病并发症  微小核糖核酸-103
英文关键词: 
基金项目:
作者单位
王奕 南京医科大学附属儿童医院检验科,江苏 南京 210008 
陈雪辉 南京市中医院检验科,江苏 南京 210001 
陈红兵 南京医科大学附属儿童医院检验科,江苏 南京 210008 
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中文摘要:
      目的 探讨血清微小核糖核酸(miRNA)-103在2型糖尿病(T2DM)前期、新诊断的T2DM以及糖尿病并发症患者中的表达水平及临床意义。 方法 选取70例研究对象,其中20例为糖耐量正常的个体(NGT组),18例为糖调节受损患者(pre-diabetes组),18例为新诊断的T2DM并无明显并发症患者(T2DM组),14例为T2DM并发症患者(T2DM伴并发症组)。所有诊断均符合美国糖尿病学会(ADA)糖尿病诊断与分型标准中的诊断标准。应用荧光定量实时 逆转录聚合酶链反应(PR-PCR)法评价各组受试者血清miRNA-103的表达水平。 结果 Pre-diabetes组、T2DM组以及T2DM并发症组血清样本中miRNA-103水平显著高于NGT组 (P<0.05);T2DM组血清样本中miRNA-103水平显著高于pre-diabetes组(P<0.05),而miRNA-103在T2DM伴并发症组与T2DM组血清中的表达差异无统计学意义(P>0.05)。 结论 血清miRNA-103有可能用于诊断及预测糖尿病的敏感的生物标志物,对于鉴别高风险患者具有至关重要的意义。
英文摘要:
      Objective To investigate the expression levels of serum miRNA-103 in pre-diabetes,newly diagnosed type 2 diabetes mellitus without evident complications and type 2 diabetes mellitus with complications. Methods Selected 70 casesin this study,there were 20 healthy controls,18 patients with impaired glucose regulation (pre-diabetes),18 newly diagnosed type 2 diabetes mellitus without evident complications and 14 type 2 diabetes mellitus with complications .They were diagnosed according to American Diabetes Association diagnostic criteria.Serum miRNA-103 expression was assessed by quantitative polymerase chain reaction. Results We found a significant increase of serum miRNA-103 expression between pre-diabetes as well as diabetic patients when both compared with controls and between diabetic patients compared to pre-diabetes(P<0.05).There was no significant difference for serum miRNA-103 expression level between diabetic patients with complications and diabetic patients without evident complications(P>0.05). Conclusions Serum miRNA-103 expression canbe a good biomarker for diagnosis and prediction of pre-diabetes and type 2 diabetes mellitus as well as for better identification of a high risk form of diabetes.
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