| 余华.骨髓间充质干细胞miRNA-196a异常表达与激素性股骨头坏死关联的临床研究[J].安徽医药,2018,22(1):68-70. |
| 骨髓间充质干细胞miRNA-196a异常表达与激素性股骨头坏死关联的临床研究 |
| A clinical analysis of the association between expression of miRNA-196a in bone marrow mesenchymal stem cells and the steroid induced femoral head necrosis |
| 投稿时间：2016-10-31 |
| DOI： |
| 中文关键词: 激素性股骨头坏死 miRNA-196a 骨髓间充质干细胞 |
| 英文关键词: steroid induced femoral head necrosis mirna-196a bone marrow mesenchymal stem cells |
| 基金项目: |
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| 摘要点击次数: 1967 |
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| 中文摘要: |
| 目的 探讨骨髓间充质干细胞miRNA-196a异常表达与激素性股骨头坏死的关联。方法 选取确诊激素性股骨头坏死患者58例及其他类型股骨头坏死患者65例作为对照；荧光标记定量PCR测定mRNA表达水平,通过问卷调查方式收集其他相关因素信息。SPSS 17.0统计软件包用于数据分析。结果 激素性股骨头坏死组骨髓间充质干细胞miRNA-196a表达水平高于对照组,且随着坏死程度的增加表达水平也随之增高(P＜0.05)；年龄大、miRNA-196a高表达,免疫性疾病家族史以及激素使用时间长均可能增加激素性股骨头坏死的发生风险,男性和体质量指数(BMI)超标则表现为弱保护因素(P＜0.05)。结论 骨髓间充质干细胞miRNA-196a异常表达与激素性股骨头坏死存在关联,且此关联受到年龄、性别以及家族史等因素的潜在影响。 |
| 英文摘要: |
| Objective To investigate the association between abnormal expression of miRNA-196a in bone marrow mesenchymal stem cells and steroid induced femoral head necrosis. Methods 58 patients with steroid induced femoral head necrosis and 65 patients with other types as controls were selected.The expression of mRNA in the femoral head was quantitatively detected by fluorescence labeling.Meanwhile,other related factors were collected via questionnaire survey.The data were analyzed with SPSS 17.0 statistical software package. Results The expression of miRNA-196a was significantly higher in steroid-induced femoral head necrosis group than that in control group,and the severer the necrosis,the higher the expression (P＜0.05).High risk of femoral head necrosis was associated with old age,higher expression of miRNA-196a,family history of immunodeficiency disease,and long-term steroid use,while men and exceeded BMI were weak protective factors (P＜0.05). Conclusions The abnormal expression of miRNA-196a in bone marrow mesenchymal stem cells is associated with steroid-induced femoral head necrosis,and this association is influenced by factors such as age,sex and family history. |
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