| 沈陈军,陈新贵,王勇.荧光偏振免疫分析法与高效液相色谱法检测环孢素A血药浓度的倒方差法Meta分析[J].安徽医药,2018,22(7):1256-1262. |
| 荧光偏振免疫分析法与高效液相色谱法检测环孢素A血药浓度的倒方差法Meta分析 |
| Comparison between FPIA and HPLC method in monitoring Cyclosporin Ablood concentration based on pearson correlation coefficient:a meta-analysis |
| 投稿时间：2017-09-30 |
| DOI： |
| 中文关键词: 荧光偏振免疫测定 色谱法, 高压液相 环孢菌素 血药浓度 统计学, 非参数 方差分析 Meta分析 |
| 英文关键词: Fluorescence polarization immunoassay Chromatography, high pressure liquid Cyclosporine Plasma concentration Statistics, nonparametric Analysis of variance Meta-analysis |
| 基金项目: |
|
| 摘要点击次数: 2351 |
| 全文下载次数: 710 |
| 中文摘要: |
| 目的 评价基于皮尔逊相关系数的荧光偏振免疫分析法(FPIA)与高效液相色谱法(HPLC)检测环孢素A(CsA)血药浓度相关性的比较研究。方法 通过文献数据库检索中国知网、维普数据库、万网数据库、Pubmed及EMbase,检索有关FPIA和HPLC测定CsA血药浓度比较研究的方法学评价文献。按照纳入和排除文献标准筛选文献,提取资料以及利用QUADAS量表进行方法学质量评价。采用Revman 5.2软件将皮尔逊相关系数Fisher’Z转化后的数据进行倒方差法Meta分析。结果 共纳入13篇文献,合计1 245例次患者检测结果。summary Fisher’Z值为1.57,95%CI为(1.31～1.83),将summary Fisher’Z值转换得出相关系数的合并效应值summary r为0.917,95%CI为(0.864～0.950),P<0.01；亚组分析提示FPIA法使用单克隆抗体或多克隆抗体试剂导致了各研究之间的高异质性(I2=95%)。纳入13篇文献其中7篇文献结论提示FPIA法测定CSA浓度较HPLC法测定值偏高,其可能是由于CsA及其代谢产物累积造成。结论 FPIA和HPLC测定CsA血药浓度结果相关性良好,两者均可作为临床常用CsA血药浓度检测方法,但FPIA法测定CsA浓度较HPLC法测定值偏高,临床解读结果时应注意检测方法的不同。 |
| 英文摘要: |
| Objective To evaluate the correlation between FPIA and HPLC method in monitoring cyclosporin concentration through the Pearson correlation coefficient by the method of inverse variance. Methods Databases including Pub Med, EMbase, CNKI, VIP and Wan Fang Data were searched to get methodological evaluation of comparison between FPIA and HPLC method in monitoring cyclosporin concentration. The studies were screened according to the inclusion and exclusion criteria. After extracting data and assessing the quality of the included studies by QUADAS, the Pearson correlation coefficient was transformed with Fisher 'Z and meta-analysis was conducted using Revman 5.2 software. Results A total of 13 studies involving 1 245 cases were included in this analysis. There was significant difference in the value of the concentration of valproic acid by FPIA and HPLC (summary fisher’Z=1.57,5%CI(1.31,1.83), summary r=0.917,5%CI(0.864,0.950),P<0.01). There was higher heterogeneity among groups(I2=95%). Sensitivity analysis showed that the result was relatively robust. Subgroup analysis showed that the use of monoclonal antibodies or polyclonal antibody reagents by FPIA resulted in heterogeneity, which could lead to high heterogeneity among studies. The 13 studies were included, of which 7 studies suggested that the concentration of CsA determined by FPIA was higher than that determined by HPLC, which may be caused by the accumulation of CSA and its metabolites. Conclusions The FPIA and HPLC method for the determination of serum concentration of cyclosporin were well correlated and used to detect the concentration of cyclosporin in clinical. The concentration of CsA determined by FPIA was higher than that measured by HPLC. The results of clinical interpretation should be paid attention to in different methods. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
