| 王伟,刘静.炎性细胞因子检测在胎膜早破合并绒毛膜羊膜炎诊断中的作用[J].安徽医药,2018,22(9):1686-1688. |
| 炎性细胞因子检测在胎膜早破合并绒毛膜羊膜炎诊断中的作用 |
| Role of inflammatory cytokine detection in the diagnosis of premature rupture of membrane complicated with chorioamnionitis |
| 投稿时间：2017-02-13 |
| DOI： |
| 中文关键词: 胎膜早破 绒毛膜羊膜炎 白细胞介素-6 白细胞介素-8 白细胞介素-10 肿瘤坏死因子-α |
| 英文关键词: Premature rupture of membrane Chorioamnionitis Interleukin-6 Interleukin-8 Interleukin-10 Tumor necrosis factor-α |
| 基金项目:河北省医学科学研究指令性课题(20130290)炎性细胞因子检测在胎膜早破合并绒毛膜羊膜炎诊断中的作用王伟,刘静 (石家庄市妇幼保健院妇产科,河北 石家庄 050000) |
|
| 摘要点击次数: 2064 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 观察炎性细胞因子检测对胎膜早破(PROM)合并绒毛膜羊膜炎的诊断价值。方法 选取石家庄市妇幼保健院2016年1～12月收治的PROM产妇100例为研究对象。依据是否合并绒毛膜羊膜炎分为炎症组(PROM合并绒毛膜羊膜炎产妇)50例和PROM组(PROM未合并破绒毛膜羊膜炎产妇)50例；炎症组产后经胎膜组织病理检查,其中轻、中度绒毛膜羊膜炎病变产妇26例,重度绒毛膜羊膜炎病变产妇24例；另选取同期胎头位妊娠正常分娩产妇50例为对照组。抽取各组产妇出现宫缩前肘静脉血,采用酶联免疫吸附法检测血清白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)含量,对比炎症组、PROM组和对照组产妇血清IL-6、IL-8、IL-10及TNF-α含量,并对炎症组不同绒毛膜羊膜炎病变程度产妇血清IL-6、IL-8、IL-10及TNF-α含量进行对比观察,分析炎性细胞因子含量对PROM合并绒毛膜羊膜炎病变程度的诊断价值。结果 炎症组血清IL-6、IL-8及TNF-α含量分别为(243.55±83.42)、(1 156.88±126.65)、(1 389.29±367.43)ng·L-1明显高于PROM组和对照组(F=91.489,P<0.01；F=477.975,P<0.01；F=18.002,P<0.01),IL-10含量为(301.93±27.61)ng·L-1明显低于PROM组和对照组(F=46.084,P<0.01);重度绒毛膜羊膜炎产妇血清IL-6、IL-8、TNF-α含量分别为(297.49±88.79)、(1 342.75±189.44)、(1 983.82±389.06) ng·L-1,轻、中度绒毛膜羊膜炎产妇分别为(191.75±66.55)、(898.64±113.20)、(1 147.78±311.56) ng·L-1,重度绒毛膜羊膜炎产妇血清IL-6、IL-8、TNF-α含量明显高于轻、中度绒毛膜羊膜炎产妇(t=4.789,P<0.01；t=9.960,P<0.01；t=8.418,P<0.01)；重度绒毛膜羊膜炎产妇血清IL-10含量为(213.33±24.45)ng·L-1,轻、中度绒毛膜羊膜炎产妇血清IL-10含量为(317.89±43.51)ng·L-1,重度绒毛膜羊膜炎产妇血清IL-10含量明显低于轻、中度绒毛膜羊膜炎产妇(t=10.577,P<0.01)。结论 炎性细胞因子检测可作为PROM合并绒毛膜羊膜炎诊断及筛查的辅助指标。 |
| 英文摘要: |
| Objective To observe the value of inflammatory cytokine detection in the diagnosis of premature rupture of membrane (PROM) complicated with chorioamnionitis.Methods A total of 100 pregnant women who were admitted in our hospital from January,2016 to December,2016 were included in the study and divided into the inflammation group(n=50,PROM complicated with chorioamnionitis) and PROM group(n=50,not complicated with chorioamnionitis). In the inflammation group,26 had mild and moderate chorioamnionitis,and 24 had severe chorioamnionitis confirmed by the pathological examination of fetal membrane.A total of 50 pregnant women with normal delivery were served as the control group.The elbow venous blood in the three groups before contraction was collected.ELISA was used to detect the serum IL-6,IL-8,IL-10 and TNF-α contents.The serum IL-6,IL-8,IL-10 and TNF-α contents in the three groups were compared.The serum IL-6,IL-8,IL-10 and TNF-α contents in the inflammation group with different chorioamnionitis lesion degrees were observed and compared in order to analyze the value of detection of the above inflammation cytokines in the diagnosis of PROM complicated with chorioamnionitis lesion degree.Results The serum IL-6,IL-8 and TNF-α contents in the inflammation group (243.55±83.42,1 156.88±126.65,and 1 389.29± 367.43 ng·L-1,respectively) were significantly higher than those in PROM group and the control group(F=91.489,P<0.01;F=477.975,P<0.01;F=18.002,P<0.01),while IL-10 level (301.93±27.61) ng·L-1was significantly lower than that in PROM group and the control group(F=46.084,P<0.01).The serum IL-6,IL-8 and TNF-α contents in the pregnant women with severe chorioamnionitis (297.49±88.79,1 342.75±189.44,and 1 983.82±389.06 ng·L-1,respectively) were significantly higher than those in the pregnant women with mild and moderate chorioamnionitis (191.75±66.55,8.64±113.20,and 1 147.78±311.56 ng·L-1,respectively)(t= 4.789,P<0.01,t=9.960,P<0.01,t=8.418,P<0.01).The serum IL-10 content in the pregnant women with severe chorioamnionitis (213.33±24.45) ng·L-1 was significantly lower than that in the pregnant women with mild and moderate chorioamnionitis (317.89±43.51) ng·L-1 (t=10.577,P<0.01).Conclusions Detection of inflammatory cytokines can be served as an assistant indicator in the diagnosis and screening of PROM complicated with chorioamnionitis. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
