文章摘要
周萍,刘娜,焦瑞宝,等.XN-3000血液分析仪三种方法检测低值血小板结果比较[J].安徽医药,2018,22(9):1694-1695.
XN-3000血液分析仪三种方法检测低值血小板结果比较
Comparison of three methods of XN-3000 hematology analyzer in detecting low-value platelet
投稿时间:2017-01-21  
DOI:
中文关键词: XN-3000血液分析仪  低值血小板  准确性
英文关键词: XN-3000 hematology analyzer  Low-level platelet  Accuracy
基金项目:
作者单位
周萍 铜陵市人民医院临床检验中心,安徽 铜陵 244009 
刘娜 铜陵市人民医院临床检验中心,安徽 铜陵 244009 
焦瑞宝 铜陵市人民医院临床检验中心,安徽 铜陵 244009 
章文 铜陵市人民医院临床检验中心,安徽 铜陵 244009 
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中文摘要:
      目的 观察XN-3000血液分析仪电阻抗法(PLT-I)和激光染色法(使用荧光染料PLT-O法和使用核酸荧光染料PLT-F法)检测低值血小板(PLT)的准确性。方法 采用PLT-I、PLT-O、PLT-F和手工法检测2015年12月至2016年5月铜陵市人民医院住院患者118例低值PLT样本,按手工法结果分为四组:PLT≤15×109·L-1组21例、15×109·L-1<PLT≤30×109·L-1组29例、30×109·L-1<PLT≤45×109·L-1组40例、45×109·L-1<PLT≤60×109·L-1组28例,每组样本仪器3种方法结果分别与手工法计数结果进行比较和相关性分析。结果 各组样本采用PLT-I、PLT-O、PLT-F检测的结果与手工法相比较,均差异无统计学意义(P>0.05);相关性分析比较,PLT-F与手工法结果相关系数(r=0.995 7)高于PLT-I与手工法相关系数(r=0.940 9)、PLT-O与手工法相关系数(r=0.962 3)。结论 XN-3000血液分析仪的3种方法检测低值PLT相比较,PLT-F与手工法结果最接近,方法准确性最高。
英文摘要:
      Objective To observe the accuracy of detecting low-level platelet (PLT-I) using X-3000 hematology analyzer (PLT-I) and laser staining (using fluorescent dye PLT-O method and nucleic acid fluorescent dye PLT-F method).Methods PLT-I,PLT-O,PLT-F and manual methods were used to detect 118 cases of low-level PLT sample who hospitalized in Tongling Peoples Hospital from December 2015 to May 2016,which were divided into 4 groups according to manual method:PLT≤15×109·L-1 group of 21 cases,15×109·L-1<PLT≤30×109·L-1 group of 21 cases,30×109·L-1<PLT≤45×109·L-1 group and 45×109·L-1<PLT≤60×109·L-1group of 28 cases.The results of 3 methods of using instrument of each group were compared with the manual method and correlative analysis was performed.Results There was no significant difference between PLT-I, PLT-O, PLT-F and manual methods(P>0.05).Correlation analysis showed that the correlation coefficient between PLT-F and manual method(r=0.995 7) was higher than that of PLT-I and manual method(r=0.940 9),and correlation coefficient between PLT-O and manual method(r=0.962 3). Conclusion The PLT-F is the closest to the manual method,and the accuracy of the method is the highest when the three methods of XN-3000 hematology analyzer are used to detect the low-value PLT.
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