| 李晓娟,史颖,杨筱凤.吉西他滨对宫颈癌患者辅助性T细胞17/CD4+CD25+调节性T细胞及肿瘤相关巨噬细胞的影响及意义[J].安徽医药,2018,22(11):2244-2249. |
| 吉西他滨对宫颈癌患者辅助性T细胞17/CD4+CD25+调节性T细胞及肿瘤相关巨噬细胞的影响及意义 |
| In fluence and significance ofgemcitabine to Th17/CD4+CD25+Treg and tumor-associated macrophage of cervical cancer |
| 投稿时间：2017-07-28 |
| DOI： |
| 中文关键词: 吉西他滨 宫颈癌 辅助性T细胞17/CD4+CD25+调节性T细胞 肿瘤相关巨噬细胞 |
| 英文关键词: Gemcitabine Cervical cancer Th17/ CD4+CD25+Treg Tumor associated macrophage |
| 基金项目:国家自然科学基金项目(81472428) |
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| 中文摘要: |
| 目的 分析吉西他滨对宫颈癌患者外周血辅助性T细胞17(Th17)/CD4+CD25+调节性T细胞(CD4+CD25+Treg)及肿瘤相关巨噬细胞的影响,为完善其药理机制提供数据支撑。方法 将陕西中医药大学第二附属医院2014年12月至2016年12月收治的59例拟行手术治疗的宫颈癌患者采用随机数字表法分为观察组30例和对照1组29例,两组均给予三维适形放疗和手术切除,观察组在此基础上给予吉西他滨新辅助化疗。另取11例同期入院体检的健康志愿者为对照2组,观察组和对照1组分别于治疗前后取患者空腹肘静脉血,对照2组于体检时取空腹肘静脉血,采用流式细胞法测定Th17与CD4+CD25+Treg的外周血细胞计数,采用酶联免疫吸附测定试剂盒测定白细胞介素-17A(IL-17A)、白细胞介素-17F(IL-17F)、白细胞介素-10(IL-10)及转化生长因子(TGF-β1)的血清水平。并于手术时留取病灶组织与癌旁健康组织,采用免疫组织化学实验测定肿瘤相关巨噬细胞表达水平。结果 (1)治疗前,观察组、对照1组和对照2组的Th17细胞计数分别为(4.75±0.63)%、(5.14±0.66)%和(12.02±1.85)%；CD4+CD25+Treg细胞计数分别为(13.41±1.52)%、(12.05±1.45)%和(7.52±0.88)%；治疗后,观察组、对照1组的Th17细胞计数分别为(16.52±3.02)%和(11.02±2.06)%,CD4+CD25+Treg细胞计数分别为(3.08±0.44)%和(6.11±0.74)%,与治疗前相比,差异有统计学意义(P<0.05)。(2)治疗后,观察组、对照1组血清IL-17A、IL-17F、IL-10及TGF-β1水平与治疗前相比,IL-17A与IL-17F水平有所升高,IL-10与TGF-β1水平有所下降,且观察组变化幅度高于对照1组,两组间差异有统计学意义(P<0.05)。 (3)观察组和对照1组病灶组织的肿瘤相关巨噬细胞表达量为(1.82±0.14)和(2.25±0.46),健康组织分别为(0.38±0.17)和(0.35±0.16),病灶组织高于健康组织,观察组高于对照1组,组间差异有统计学意义(P<0.05)。结论 吉西他滨能够抑制宫颈癌患者Th17/CD4+CD25+Treg漂移和肿瘤相关巨噬细胞活性,可能是其发挥抗癌作用的重要机制之一。 |
| 英文摘要: |
| Objective To analyze the influence and significance of gemcitabine (GEM) to Th17/CD4+CD25+Treg and tumor-associated macrophage of cervical cancer,so as to provide data evidence for improving its pharmacological mechanism.Methods Totally 59 patients with cervical cancer and prepared for surgery admitted to The Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine from December 2014 to December 2016 were assigned into 2 groups randomly:30 in the observation group and 29 in the first control group.All the patients were given 3D conformal radiation therapy (CRT) and exairesis,at the same time,patients of the observation group were given GEM before surgery.Another 11 healthy volunteers who were admitted for physical examination at the same time were taken as the second control group.The fast cubital venous blood of patients in the observation group and the first control group was extracted both before and after treatment,and the fast cubital venous blood of the second control group was extracted during physical examination.Th17 cell count and CD4+CD25+Treg cell count were both detected by flow cytometry method,and the levels of interleukin 17A (IL-17A),interleukin 17F (IL-17F),interleukin 10 (IL-10) and transforming growth factor beta 1 (TGF-β1) were detected by ELISA kits.Furthermore,both the lesion and healthy tissues were extracted to detect tumor-associated macrophage expression by immunohistochemistry assay.Results (1) Before treatment,the Th17 cell counts of the observation group,the first control group and the second control group were (4.75±0.63)%,(5.14±0.66)% and (12.02±1.85)%,and the CD4+CD25+Treg cell counts were (13.41±1.52)%,(12.05±1.45)% and (7.52±0.88)%.After treatment,the Th17 cell counts of the observation group and the first control group were (16.52±3.02)% and (11.02±2.06)%,and the CD4+CD25+Treg cell counts were (3.08±0.44)% and (6.11±0.74)%,which compared to before treatment,had statistically significant differences (P<0.05).(2) In the observation group and the first control group,after treatment,the IL-17A and IL-17F levels were increased,while IL-10 and TGF-β1 levels were decreased when compared to those before treatment,and the variation amplitude of the observation group was higher than that of the first control group;the difference had statistical significance (P<0.05).(3) The expressions of tumor associated macrophage in lesion tissues of the observation group and the first control group were (1.82±0.14) and (2.25±0.46),and in healthy tissues of the observation group and the first control group were (0.38±0.17) and (0.35±0.16).The macrophage expressions in lesion tissues were higher than those in healthy tissues,and the expressions of the observation group was higher than those of the first control group;the differences between groups were statistically significant (P<0.05).Conclusion gemcitabine could control Th17/CD4+CD25+Treg drifting and tumor-associated macrophage activity,which may be one of its important pharmacological mechanisms to treat cervical cancer. |
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