| 聂万松,汪艳,占晓敏,等.液相色谱法测定筋骨草胶囊特征图谱研究[J].安徽医药,2019,23(1):46-49. |
| 液相色谱法测定筋骨草胶囊特征图谱研究 |
| Study on determination of the fingerprint characteristics of Jingucao capsule by HPLC |
| 投稿时间：2017-07-14 |
| DOI： |
| 中文关键词: 色谱法,高压液相 白毛夏枯草 木犀草素 |
| 英文关键词: Chromatography,high pressure liquid Ajuga degumbens Luteolin |
| 基金项目: |
|
| 摘要点击次数: 2027 |
| 全文下载次数: 529 |
| 中文摘要: |
| 目的 建立液相色谱法测定筋骨草胶囊特征图谱鉴别的方法,完善筋骨草胶囊质量标准。方法 采用Kromasil 100-5C18 (250 mm×4.6 mm,5 μm)色谱柱,流动相:乙腈为流动相A,0.4%磷酸溶液为流动相B,进行梯度洗脱,检测波长:350 nm,流速:1 mL/min,柱温30 ℃。结果 在供试品特征图谱中选定的3个特征峰中,与木犀草素参照物峰1(S),计算出的峰2、3与S峰的相对保留时间为:1.218 (峰2)、1.794 (峰3)。结论 本法专属性强,结果准确,操作方便,可作为本品的质量控制方法。 |
| 英文摘要: |
| Objective To establish a method for the identification of the fingerprint characteristics of Jingucao capsule by HPLC to improve the quality standard.Methods The procedure was performed on the Kromasil 100-5C18 (250 mm×4.6 mm,5 μm) column,at 30℃,detection at 350nm,with acetonitrile as mobile phase A,0.4% phosphoric acid solution as mobile phase B,gradient elution,flow rate:1 mL·min-1.Results Among the selected characteristic peaks,the relative retention times of peaks 2,3 and Speaks were 1.218(peak 2) and 1.794 (peak 3) in the selected 3 characteristic peaks of luteolin reference 1 (S).Conclusion This method is specific,accurate and convenient to operate.It can be used as a quality control method for this product. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
