| 邱涵,吕晓君.石斛枸杞颗粒对小鼠免疫功能的影响[J].安徽医药,2019,23(3):458-462. |
| 石斛枸杞颗粒对小鼠免疫功能的影响 |
| Experimental study on enhancing immunity function of dendrobium medlar granules in mice |
| 投稿时间：2017-10-16 |
| DOI： |
| 中文关键词: 石斛枸杞颗粒小鼠的细胞免疫、单核巨噬细胞吞噬功能和NK细胞免疫功能均有不同程度的增强作用。 关键词:石斛枸杞颗粒 免疫功能 小鼠 |
| 英文关键词: Dendrobium Medlar Granules Immunity Function Mice |
| 基金项目: |
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| 中文摘要: |
| 目的 研究石斛枸杞颗粒对小鼠免疫功能的影响。 方法 160只小鼠采用随机数字表法分为阴性对照组、石斛枸杞颗粒高、中、低剂量组(7.5 g/kg BW、5.0 g/kg BW、2.5 g/kg BW),每组40只,连续灌胃30 d,每日1次。采用耳肿胀法观察其对小鼠细胞免疫功能的影响；检测血清溶血素水平、抗体生成细胞数观察其对小鼠体液免疫功能的影响；采用小鼠腹腔巨噬细胞吞噬鸡红细胞实验观察其对小鼠吞噬功能的影响；采用乳酸脱氢酶(LDH)测定法观察其对小鼠NK细胞活性的影响。 结果与阴性对照组比较,石斛枸杞颗粒中、低剂量组能明显促进小鼠迟发型变态反应(P<0.05)、高剂量组可提高小鼠巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数(P<0.05),高、中剂量组可显著增强NK细胞活性(P<0.05)；但各剂量组对小鼠体质量、胸腺/体质量比值、脾脏/体质量比值、抗体生成细胞数、血清溶血素水平无明显影响(P＞0.05)。 |
| 英文摘要: |
| Objective To study the impact of the Dendrobium Medlar Granules on immunity function in mice. Methods 160 mice were randomly divided into 4 groups:negative control group and the high,the medium and the low dosage groups of CPC (7.5 g/kg BW,5.0 g/kg BW,2.5 g/kg BW).Each mouse was orally administered once a day for 30 days.After the end of the administration,the cellular immunity activity in mice was tested using delayed type hypersensitivity experiment.The serum hemolysin level and antibody producing cell count were tested to observe the humoral immune effects.The peritoneal macrophage phagocytic functjon in mice was carried out by detecting macrophage phagocytosis of chicken erythrocytes capacity.And the NK cell activity were tested by detecting the level of LDH.Results Compared with the negative control group,the medium and low dosage groups of Dendrobium Medlar Granules could significantly strengthen the delayed type hypersensitivity of mice (P<0.05).The high dosage groups of Dendrobium Medlar Granules could significantly increase macrophage phagocytosis of chicken erythrocytes capacity (P<0.05).The high and medium dosage groups of Dendrobium Medlar Granules could significantly strengthen NK cells activity of mice (P<0.05).However,Dendrobium Medlar Granules had no significant effects on body weight increase,ratio of spleen weight to body weight,ratio of thymus weight to body weight,level of serum hemolysin and antibody producing cell count of mice (P＞0.05). ConclusionDendrobium Medlar Granules may increase immunological functions to different degrees in mice,including cellular immune,peritoneal macrophage phagocytic functjon and NK cell activity. |
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