文章摘要
秦学玲,康绍建,马娜,等.丹参片的超高效液相色谱指纹图谱[J].安徽医药,2019,23(8):1516-1519.
丹参片的超高效液相色谱指纹图谱
Studies on the UPLC fingerprints of Danshen tablets
投稿时间:2018-03-20  
DOI:
中文关键词: 色谱法,高压液相  丹参  指纹图谱
英文关键词: Chromatography,high pressure liquid  Salvia miltiorrhiza  Fingerprint
基金项目:
作者单位
秦学玲 云南省食品药品监督检验研究院中药检验所,云南 昆明 650106 
康绍建 云南省食品药品监督检验研究院中药检验所,云南 昆明 650106 
马娜 云南省食品药品监督检验研究院中药检验所,云南 昆明 650106 
蒋新平 云南百康药业有限公司,云南 昆明 650106 
杨璐璐 解放军联勤保障部队第九二〇医院中医科,云南 昆明 650032 
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中文摘要:
      目的 运用超高效液相色谱法对丹参片进行指纹图谱研究,为全面评价丹参片的整体质量提供参考。方法 采用UPLC法,色谱柱为Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7 μm),以0.2%磷酸溶液(A)-乙腈(B)为流动相,梯度洗脱(0[KG-*3]~10 min,4%B→18%B;10[KG-*3]~15 min,18%B→33%B;15[KG-*3]~19 min,33%B→49%B;19[KG-*3]~37 min,49%B→79%B);柱温19 ℃,样品管理器温度3.5 ℃;流速为0.25 mL/min;以丹酚酸B为参照峰,检测波长280 nm。结果 建立了丹参片的UPLC指纹图谱共有模式,确定了13个峰为共有峰,所有样品的相似度均在0.900以上;指认了其中的11个共有峰,分别为:丹参素钠、原儿茶醛、迷迭香酸、紫草酸、丹酚酸B、丹酚酸A、二氢丹参酮、隐丹参酮、丹参酮Ⅰ、熊果酸和丹参酮ⅡA。结论 所建立的方法能在30 min内检测丹参片中的多种成分,操作简便、快速,能全面控制丹参片的整体质量。
英文摘要:
      Objective To establish a UPLC method for fingerprints analysis of Danshen tablets,and provide reference for comprehensive evaluation of Danshen tablets.Methods UPLC method was adopted.The determination was performed on Waters ACQUITY UPLC BEH C18 (2.1 mm×50 mm,1.7 μm) column with mobile phase consisted of 0.2% Phosphoric acid(A)-acetonitrile(B) by a gradient eluted program(0[KG-*3]~10 min,4%B→18%B;10[KG-*3]~15 min,18%B→33%B;15[KG-*3]~19min,33%B→49%B;19[KG-*3]~37 min,49%B→79%B)with a flow rate of 0.25 mL/min at 19℃.Sample manager temperature is 3.5 ℃.The detection wavelength was 280 nm with salvianolic acid Bas the reference peak.Restults The standard fingerprint was established,and the overall similarity of all samples exceeded 0.900.Moreover,13 common peaks were found.Among them,11 peaks were identified,including tanshinol,protocatechuic aldehyde,rosmarinci acid,lithospermic acid,salvianolic acid B,salvianolic acid A,dihydrotanshinone,cryptotanshinone,tanshinone Ⅰ,ursolic acid,tanshinone ⅡA.Conclusion This method is simple,rapid,and can be used to detect the multiple active ingredients in Danshen tablets within 30min.So it can be used to evaluate the quality uniformity of Danshen tablets.
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