| 张丽华,刘裕,赵鹏,等.化香树果序多糖脱蛋白工艺研究[J].安徽医药,2019,23(9):1711-1714. |
| 化香树果序多糖脱蛋白工艺研究 |
| Study on deproteinization of polysaccharides from Platycarya strobilacea Sieb.et Zucc |
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| DOI：10.3969/j.issn.1009-6469.2019.09.004 |
| 中文关键词: 化香树果序 多糖 木瓜蛋白酶 脱蛋白 |
| 英文关键词: Platycarya strobilacea Sieb.et Zucc Polysaccharides Papain Deproteinization |
| 基金项目:陕西省自然科学基金(2019JM502)；陕西省中医管理局中医药科研课题(ZYPT012)；陕西省教育厅自然专项(14JK1194)； |
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| 中文摘要: |
| 目的 应用木瓜蛋白酶法脱除化香树果序多糖中蛋白，探究最佳工艺条件。方法 通过对酶用量、酶作用时间、酶作用温度及pH值四个单因素考察，采用响应面优化法试验分析。结果 最佳工艺条件，木瓜蛋白酶用量1.508%，温度65 ℃，酶解时间92.3 min，pH＝7.0，多糖蛋白脱除率为90.90%，多糖保留率为84.06%。结论 木瓜蛋白酶法是一种较优的多糖脱除蛋白方法。 |
| 英文摘要: |
| Objective To screen the optimum method for deproteinization of Platycarya strobilacea Sieb.et Zucc polysaccharides.Methods In this study，the optimum conditions for deproteinized papain were studied by four single factors，enzyme dosage，enzyme action time，enzyme temperature and pH，and response surface optimization and orthogonal experiment.Results The optimum process condition was that the papain 1.508%，65 ℃，92.3 min，and pH＝7.The rate of the removal from polysaccharides protein was maximum，which was 90.90%，and the retention rate of polysaccharides was excellent，which was 84.06%.Conclusion The papain-deproteinization method was the best for protein removal from Platycarya strobilacea Sieb.et Zucc polysaccharides. |
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