| 周芮萱,马松涛,蒋刚.三叶苷对 N?甲基?D?天冬氨酸诱导的人神经母细胞瘤细胞损伤的保护作用[J].安徽医药,2019,23(10):1926-1929. |
| 三叶苷对 N?甲基?D?天冬氨酸诱导的人神经母细胞瘤细胞损伤的保护作用 |
| The protective effects of Trilobatin on SH?SY5Y cell injury induced by NMDA |
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| DOI：10.3969/j.issn.1009?6469.2019.10.005 |
| 中文关键词: 壳斗科 神经母细胞瘤 N?甲基天冬氨酸 印迹法，蛋白质 三叶苷 人神经母细胞瘤细胞 |
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| 中文摘要: |
| 目的研究三叶苷( Trilobatin)对 N?甲基 ?D?天冬氨酸( N?methyL?D?aspartate，NMDA)诱导的人神经母细胞瘤细胞( SH? SY5Y)损伤的保护作用。方法采用体外细胞培养的方法，建立 SH?SY5Y细胞 NMDA损伤模型。分设对照组( 0 mmol/L NMDA)、 NMDA损伤模型( 0 μmol/L三叶苷)组、三叶苷组( 10，50，100 μmol/L)。对照组正常培养， NMDA损伤模型组以 2 mmol/L的 NMDA为造模浓度，三叶苷组在 NMDA损伤模型组的基础上分别以 10，50，100 μmol/L的三叶苷为细胞活性测试浓度。采用四甲基偶氮唑盐微量酶反应比色法( MTT法)测定三叶苷对 SH?SY5Y细胞活性的影响；通过 MTT法、乳酸脱氢酶(LDH)释放量检测三叶苷对 NMDA诱导 SH?SY5Y细胞损伤的保护作用，用蛋白质印迹法( Western Blot)检测细胞内促凋亡蛋白 Bax，抗凋亡蛋白 Bcl?2及 NMDA受体调节亚单位 NR2B蛋白表达水平，研究其作用机制。结果三叶苷在 1～100 mmoL/L范围内不影响 SH?SY5Y细胞的存活率。与对照组相比， NMDA损伤模型组细胞存活率为( 53.13±3.31)%，细胞存活率明显降低，细胞凋亡明显增高。与对照组相比，三叶苷组( 10，50，100 μmol/L)细胞存活率分别为( 56.28±2.05)%、(68.64±1.74)%及 |
| 英文摘要: |
| Objective To study the protective effects of Trilobatin on SH?SY5Y cell injury induced by N?methyL?D?aspartate(NMDA).Methods NMDA induced SH?SY5Y cells injury model was established by in vitro cell culture and was assigned in to control group(0 mmol/L NMDA)，NMDA injury model group(0 μmol/L Trilobatin)，Trilobatin(10，50，100 μmol/L)group.The control group was cultured normally，the NMDA injury model group used 2 mmol/L of NMDA as the model?making concentration， and the Trilobedin group used 10，50，100 μmol/L of Trilobedin as the cell activity test concentration on the basis of NMDA injurymodel group.MTT method was used to detect cell activity in order to determine the effective concentration.MTT assay and LDH as?say were used to confirm the protective effects of Trilobatin.The protein expression levels of Bax，Bcl?2 and NR2B were detected by Western blotting.Results Trilobatin could not reduce the survival rate of SH?SY5Y cells at the concentration from 1 to 100 mmol/L.Compared with the control group，NMDA significantly reduced the cell viability，the cell viability decreased to(53.13±3.31)%， the cell apoptosis was significantly increased.Compared with the control group，the cell viability rate of pretreatment with different concentration of Trilobatin(10，50，100 μmol/L)groups were(56.28±2.05)%，(68.64±1.74)% and(75.98±1.84)%，respectively， the cell viability significantly increased(F＝293.3，P＜0.001)，the LDH release decreased and apoptosis decreased significantly |
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