| 姚琳琳,杨丽,王华莉.小檗碱改善多囊卵巢综合征大鼠卵巢功能及其机制[J].安徽医药,2019,23(10):1930-1934. |
| 小檗碱改善多囊卵巢综合征大鼠卵巢功能及其机制 |
| Preliminary study on berberine improving ovarian function and mechanism in rats with polycystic ovary syndrome |
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| DOI：10.3969/j.issn.1009?6469.2019.10.006 |
| 中文关键词: 小檗碱 多囊卵巢综合征 睾酮 黄体激素类 瘦素 促性腺素类 胰岛素 |
| 英文关键词: Berberine Polycystic ovary syndrome Testosterone Corpus luteum hormones Leptin Gonadotropins Insulin |
| 基金项目: |
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| 摘要点击次数: 1906 |
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| 中文摘要: |
| 目的研究小檗碱对多囊卵巢综合征大鼠模型血清胰岛素水平及卵巢组织中胰岛素受体(IR)表达影响。方法构建多囊卵巢综合征大鼠模型， SD大鼠分为:正常组、模型组、小檗碱组(终浓度为 200 mg/kg的小檗碱灌胃)、二甲双胍组(终浓度为 170 mg/kg的二甲双胍灌胃)，每组 20只。测定大鼠体质量和卵巢、子宫质量， HE染色法测定卵巢组织中成熟卵泡及黄体数，酶联免疫吸附法(ELISA)测定血清中黄体生成素(LH)、促卵泡生成素(FSH)、睾酮、瘦素(Leptin)、胰岛素水平， qPCR测定卵巢组织中胰岛素样生长因子(I IGF?I)、瘦素、胰岛素样生长因子 I受体(IGF?IR)、 IR mRNA相对表达量水平。结果模型组多囊卵巢综合征大鼠体质量和卵巢质量明显升高，成熟卵泡数明显降低，子宫质量和黄体数减少，同时血清中 LH、FSH、睾酮、瘦素水平升高，胰岛素水平( 4 569.81±528.96)pg/mL也升高，卵巢组织中 IGF?I、瘦素、 IGF?IR升高， IR mRNA相对表达量(1.95±0.12)水平升高，与正常组大鼠相比，均差异有统计学意义(P＜0.05)。小檗碱组灌胃以后，大鼠体质量和卵巢质量明显降低，成熟卵泡数明显升高，子宫质量和黄体数增多，同时血清中 LH、FSH、睾酮、瘦素水平降低，胰岛素水平［(2 741.21±247.68)pg/mL］也降低，卵巢组织中 IGF?I、瘦素、 IGF?IR、IR mRNA相对表达量( 1.62±0.10)水平降低，与模型组大鼠相比，差异有统计学意义( P＜0.05)；与二甲双胍组差异无统计学意义(P＞0.05)。结论小檗碱可以改善多囊卵巢综合征大鼠激素分泌，调节胰岛素水平，促进排卵，改善卵巢功能。 |
| 英文摘要: |
| Objective To explore the effect of berberine on serum insulin level and the expression of insulin receptor(IR)in the ovarian tissue of the rat model with polycystic ovary syndrome.Methods A rat model of polycystic ovary syndrome was construct? ed.SD rats were assigned into normal group，model group，berberine group(intragastric administration of berberine at a final con? centration of 200 mg/kg)，metformin group(intragastric administration of metformin at a final concentration of 170 mg/kg)，with 20 rats in each group.The body mass and quality of ovary and uterus was measured.The number of mature follicles and corpus luteumin ovarian tissue was determined by HE staining.Serum luteinizing hormone(LH)，follicle stimulating hormone(FSH)，testoster? one，leptin(Leptin)，insulin levels were determined by enzyme?linked immunosorbent assay(ELISA).The relative expression level of insulin?like growth factor I(IGF?I)，leptin，insulin?like growth factor I receptor(IGF?IR)and IR mRNA in ovarian tissue was determined by qPCR.Results In the model group，the body mass and ovarian quality of the rats with polycystic ovary syndrome were significantly increased，the number of mature follicles was significantly decreased，and the uterus mass and the number of cor? pus luteum were decreased.The levels of serum LH，FSH，testosterone and leptin were elevated，and the insulin level was also in? creased to(4 569.81±528.96)pg/mL.The levels of IGF?I，leptin，IGF?IR，IR mRNA relative expression［( 1.95±0.12)］ were in? creased；compared with the normal group，the difference was statistically significant(P＜0.05).After berberine was administered，in the berberine group，the body mass and ovarian quality of the rats were significantly decreased，the number of mature follicles was significantly increased，the uterus quality and the number of corpus luteum increased.Meanwhile the levels of serum LH，FSH，tes? tosterone and leptin were decreased，and insulin level was also decreased to(2 741.21±247.68)pg/mL.The expressions of IGF?I， leptin，IGF?IR and IR mRNA relative expression［(1.62±0.10)］in ovarian tissue were decreased；compared with the model group， the difference was statistically significant(P＜0.05).Compared with the metformin group，the difference was not statistically signifi? cant(P＞0.05).Conclusion Berberine can improve the level of hormone，regulate insulin level，promote ovulation，and improve the function of the ovary in rats with polycystic ovary syndrome. |
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