| 周孟,廖祥明,王珊,等.槲皮素抑制人肝癌细胞 HepG2的体内外活性研究[J].安徽医药,2019,23(11):2136-2141. |
| 槲皮素抑制人肝癌细胞 HepG2的体内外活性研究 |
| In vivo and in vitro anticancer activity of quercetin against human liver cancer HepG2 Cells |
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| DOI：10.3969/j.issn.1009?6469.2019.11.005 |
| 中文关键词: 槲皮素 肝癌 HepG2细胞 抗肿瘤活性 细胞凋亡 |
| 英文关键词: Quercetin Liver cancer HepG2 cell Anticancer activity Cell apoptosis |
| 基金项目:贵州省民族药药效物质基础研究科技创新人才团队\贵州省高层次创新型人才培养(百层次)(黔科合平台人才［ 2016］5613\5677) |
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| 中文摘要: |
| 目的系统评价槲皮素( Qu)体内外干预 HepG2人肝癌细胞增殖效果。方法使用 MTT法检测不同浓度 Qu在不同作用时间对 HepG2细胞的体外抑制活性；采用裸鼠移植瘤实验，评价腹腔注射 Qu对 HepG2人肝癌裸鼠移植瘤生长的抑制作用；使用 TUNEL法检测凋亡指数。结果 Qu作用 HepG2细胞 24、48和 72 h的 IC50分别为 110.50，63.73和 46.38 μmol/L；12.5，25和 50 mg/kg的 Qu都能显著抑制裸鼠皮下移植瘤的生长，且呈浓度依赖性；其中 50 mg/kg的 Qu对人肝癌 HepG2细胞裸鼠皮下肿瘤模型的抑瘤率为 46.65%，差异有统计学意义( P＜0.05)，相对肿瘤增殖率为 46.68%。同时 Qu还会导致约 20.17%的体内肿瘤细胞发生凋亡，且几乎无毒性，表现出良好的抑瘤效果。结论 Qu能较为显著的抑制体内外 HepG2肝癌细胞的增殖并会导致其发生凋亡，且由于极低的毒性及在自然界的广泛分布，使 Qu成为新型抗肝癌药物开发的重要资源。 |
| 英文摘要: |
| Objective To evaluate the anticancer effect of quercetin(Qu)against HepG2 cells in vitro and in vivo.Methods MTT assay was used to evaluate the anticancer activity of Qu against HepG2 cells in vitro，and nude mice xenograft model was employedto study the in vivo antitumor activity with intraperitoneal injection of Qu.TUNEL assay was used to test the apoptosis index of thetumor issue.Results The IC50 values of Qu against HepG2 in vitro with experimental time of 24，48 and 72 h were 110.50，63.73 and 46.38 μmol/L，respectively.All three concentrations of Qu(12.5，25 and 50 mg/kg)could inhibit the growth of subcutaneous xenografts in Balb/C mice in a concentration?dependent manner.Among them，the tumor inhibition rate of Qu with concentration of 50 mg/kg was 46.65%，which had statistically significant effect(P＜0.05)，and the relative proliferation rate was 46.68%.Meanwhile，nearly 20% of tumor cell apoptosis and no toxicity were observed，suggesting a good inhibitory effect of Qu against liver cancer.Conclusion Qu cansignificantly inhibit the proliferation of HepG2 cells in vitro and in vivo and cause tumor cells apoptosis.Due to the low toxicity andwidespread distribution in nature，Qu is an important resources for developing novel anti?hepatocarcinoma drugs. |
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