| 赵杰,吴繁荣,李宁,等.泽漆部分化学成分研究[J].安徽医药,2019,23(11):2142-2145. |
| 泽漆部分化学成分研究 |
| Study on chemical constituents of Euphorbia helioscopia L |
| |
| DOI：10.3969/j.issn.1009?6469.2019.11.006 |
| 中文关键词: 泽漆 核磁共振，生物分子 酶联免疫吸附测定 辛卡利特 化学成分 结构鉴定 银屑病 |
| 英文关键词: Euphorbia helioscopia L. Nuclear magnetic resonance,biomolecular Enzyme?linked immunosorbent assay Sincalide Chemical constituents Structure identification Psoriasis |
| 基金项目: |
|
| 摘要点击次数: 2338 |
| 全文下载次数: 728 |
| 中文摘要: |
| 目的研究大戟属植物泽漆的化学成分，并对分离得到的单体化合物进行初步抗银屑病活性筛选。方法泽漆全草的 90%乙醇提取物经石油醚和氯仿萃取粗分离，再分别经过硅胶柱层析、 Sephadex LH?20柱层析、 ODS?C18柱层析，薄层色谱检识及重结晶等方法进行系统分离和纯化，并通过核磁共振氢谱、核磁共振碳谱、二维核磁共振、质谱等光谱学技术进行结构鉴定。应用人胆囊收缩素 /缩胆囊素八肽( CCK?8)法体外检测单体化合物对人角质形成细胞( HaCaT)的抑制作用，酶联免疫吸附测定(Elisa)法检测单体化合物的抗炎活性。结果从石油醚部位和氯仿部位分离纯化得到 6个化合物，分别为豆甾 ?5?烯?3β，7α?二醇， 24R?环阿屯 ?25?烯?3β，24?二醇， 24S?环阿屯 ?25?烯?3β，24?二醇，阿替生烷 ?3β，16α，17?三醇， 3，12?dihydroxy? clerodan?13E?en |
| 英文摘要: |
| Objective To investigate the chemical constituents of Euphorbia helioscopia L and their anti?psoriasis activities.Meth? ods The separation and purification of constituents were performed by silica gel，Sephadex LH?20，MCI，ODS?C18 and recrystalli?zation.Their structures were elucidated by spectroscopic methods.The effects of these constituents on HaCaT Cell proliferation weremeasured by CCK?8 assay.And their anti?inflammatory effects were measured by Elisa.Result Six compounds were isolated and their structures were identified as stigmast?5?en?3β，7α?diol，(24R)?cycloart?25?ene?3β，24?diol，(24S)?cycloart? 25?ene?3β，24?di? ol，ent?atisane?3β，16α，17?triol，3，12?dihydroxyclerodan?13E?en?15?oic acid，ingenol.Conclusion Compounds stigmast?5?en?3β，7α ?diol was isolated from this genus for the first time.Compounds(24R)?cycloart?25?ene?3β，24?diol，(24S)?cycloart? 25?ene?3β，24? diol，ent?atisane?3β，16α，17?triol，3，12?dihydroxyclerodan?13E?en?15?oic acid，ingenol were isolated from Euphorbia helioscopia L for the first time.Compounds stigmast?5?en?3β，7α?diol，ent?atisane?3β，16α，17?triol，3，12?dihydroxyclerodan?13E?en?15?oic acid， ingenol were potent inhibitors against HaCaT cells in vitro and were capable of decreasing IL?6 in TNF?α stimulated HaCaT cellsin vitro. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
