| 张珊珊,蒲超,张翼,等.微小 RNA?503在急性脑梗死病人血清中的表达及对胰岛素样生长因子 1受体信号通路的调控[J].安徽医药,2019,23(11):2146-2151. |
| 微小 RNA?503在急性脑梗死病人血清中的表达及对胰岛素样生长因子 1受体信号通路的调控 |
| The expression of serum microRNA?503 in patients with acute cerebral infarction and its effection on insulin?like growth factor?1 receptor signaling pathway |
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| DOI：10.3969/j.issn.1009?6469.2019.11.007 |
| 中文关键词: 脑梗死 microRNA?503 胰岛素样生长因子 1受体 信号通路 的表， |
| 英文关键词: Cerebral infarction MicroRNA?503 Insulin?like growth factor?1 receptor Signaling pathway |
| 基金项目:国家自然科学基金资助项目( 81870966)；南充市科技局课题( KY?16YFZJ0012)；南充市市校合作课题( NSMC20170454) |
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| 摘要点击次数: 2013 |
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| 中文摘要: |
| 目的探究微小 RNA?503(miRNA?503)在脑梗死病人血清中的表达及对胰岛素样生长因子 1受体( IGF?1R)信号通路的调控。方法选择 2017年 9月至 2019年 4月南充市中心医院确诊急性脑梗死病人 92例作为观察组及同期体检健康者 92例作为对照组，采用实时荧光定量聚合酶链式反应( qPCR)方法对比两组病人血清中 miRNA?503的表达水平；酶联免疫吸附试验(ELISA)检测两组血清样本中 IGF?1R的表达水平。 qPCR方法检测人脑正常胶质细胞 HEB及胶质瘤细胞 U87中 miRNA?503的表达水平，蛋白质免疫印迹( WB)检测两组细胞中 IGF?1R的表达水平。将 U87细胞分为 3组:不做处理的空白对照组，转染 miRNA?503模拟物的 miRNA?503模拟组及转染阴性对照物的阴性对照组，流式细胞术检测 3组细胞的凋亡情况； qPCR方法检测 3组细胞中 miRNA?503的表达水平； WB检测 3组细胞中 IGF?1R、p?Akt、Bcl?xl、Bax、c?Myc、p?PI3K的蛋白表达水平。结果 miRNA?503在观察组血清及 U87细胞中的表达量( 0.37±0.09)显著低于对照组( 1.24±0.31)和 HEB细胞( 1.58±0.28)(P＜ 0.05)； IGF?1R在观察组血清( 3.67±0.41)ng/mL及 U87细胞中的表达量( 0.93±0.11)ng/mL显著高于对照组( 1.98±0.20)ng/mL和 HEB细胞( 0.57±0.06)ng/mL(P＜0.05)。空白对照组细胞、阴性对照组细胞及 miRNA?503模拟组细胞增殖差异有统计学意义( P＜0.05)与空白对照组相比， miRNA?503模拟组细胞增殖率显著降低( P＜0.05)。 miRNA?503、c?Myc、Bax在 miRNA? 503模拟组中达水平显著高于空白对照组( P＜0.05)； IGF?1R、p?Akt、Bcl?xl、p?PI3K在 miRNA?503模拟组中的表达水平显著低于空白对照组( P＜0.05)。结论在急性脑梗死病人血清中， miRNA?503呈现高水平表达，而 IGF?1R则呈现低水平表达。 miRNA?503在 U87细胞中的过表达能够抑制细胞增殖，可能是通过抑制 IGF?1R表达，进而调节 PI3K/Akt通路中相关蛋白表达，从而抑制了 U87的增殖。 |
| 英文摘要: |
| Objective To study the expression of serum microRNA?503(miRNA?503)in patients with acute cerebral infarction and its effection on insulin?like growth factor?1 receptor(IGF?1R)signaling pathway.Methords 92 cases patients with acute cere? bral infarction(observation group)and healthy control(control group)were selected to compare their expression of serum miRNA? 503 by quantitative real?time PCR(qPCR)and IGF?1R by enzyme?linked immunosorbent assay(ELISA).The expression of miRNA?503 in gliocyte cells(HEB cells)and glioma cell(U87 cells)was detected by qPCR.The expression of IGF?1R in both cells was detected by Western blot(WB).Then the U87 cells were divided into 3 groups:untreated cells as blank group，cells transfected with miRNA?503 mimics as miRNA?503 mimics group，cells transfected with negative control as negative control group.Cell apopto?sis in 3 groups was detected by Flow cytometric method；The expression of miRNA?503，IGF?1R，p?Akt，Bcl?xl，Bax，c?Myc and p? PI3K in 3 groups were detected.Results The expression of miRNA?503 in observation group and U87 cells were much lower than that in control group and HEB cells(P＜0.05).The expression of IGF?1R in observation group and U87 cells were much lower than that in control group and HEB cells(P＜0.05).There was a significant difference of cell proliferation among 3 groups(F＞ 0.05).The cell proliferation in miRNA?503 minics group was much lower than the blank control group(P＜0.05).The expression of miRNA?503，c?Myc，and Bax in miRNA?503 minics group were much higher than the blank control group(P＜0.05)，the expres? sion of IGF?1R，p?Akt，Bcl?xl，Bax，and p?PI3K were much lower(P＜0.05).Conclusion The expression of serum miRNA?503 in patients with acute cerebral infarction was low，however，the IGF?1R was high.Overexpressin of miRNA?503 could induce apoptosis of U87 cells，which may inhibit the expression of IGF?1R，and further regulate the PI3K/Akt signaling pathway. |
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