| 杨博元,马勇,郭杨,等.肌内注射骨折合并脑外伤大鼠血清治疗单纯骨折的放射学及组织形态学观察[J].安徽医药,2020,24(1):111-114. |
| 肌内注射骨折合并脑外伤大鼠血清治疗单纯骨折的放射学及组织形态学观察 |
| YANG Boyuan1，MA Yong1，GUO Yang1，ZHU Yaliang2，HUANG Hao2，ZHU Aihong2，FANG Ying2 |
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| DOI：10.3969/j.issn.1009?6469.2020.01.028 |
| 中文关键词: 股骨骨折 /药物疗法 注射，肌肉内 血清 颅脑损伤 骨折愈合 骨痂 骨生成 骨密度 大鼠， Sprague?Dawley |
| 英文关键词: Femoral fractures/drug therapy Injections,intramuscular Serum Craniocerebral trauma Fracture healing Bony callus Osteogenesis Bone density Rats,Sprague?Dawley |
| 基金项目:江苏省金坛市科技计划社会发展项目( JT2013070，JT2014061) |
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| 中文摘要: |
| 目的观察对单纯股骨骨折大鼠肌内注射骨折合并脑外伤大鼠血清(“促骨折愈合血清”)后的放射学及组织形态学变化，以确定该血清是否具备有加速骨折愈合作用。方法 2017年 3月至 2018年 3月，制备骨折合并脑外伤模型 SD大鼠 20只经腹主动脉采血，并分期制备治疗血清；选取 20只健康大鼠提取阴性对照血清，全部加标记并冷藏。应用随机数字表进行分组，每组 32只，分为实验组，对照组及空白组， 96只大鼠均为股骨中段骨折。肌内注射治疗血清用于实验组，肌内注射阴性对照血清用于对照组，空白组肌内注射生理盐水。并于术后第 1周、 2周、 3周、 4周各处死 8只大鼠，利用放射分析比较三组骨折的愈合情况: X射线照射、双能 X线骨密度测量和组织形态学观察。结果术后 X线结果提示实验组骨折愈合进程较对照组、空白组明显加快。测量骨痂体积发现:术后第 2周，实验组骨痂体积( 30.44±2.33)mm3，对照组骨痂体积( 22.68±1.95)mm3，空白组骨痂体积( 21.66±2.35)mm3对照组和空白组骨痂体积明显小于实验组( P＝0.000)；术后第 3周，实验组骨痂体积( 26.46± 1.58)mm3对照组骨痂体积( 27.2，9±1.66)mm3空白组骨痂体积( 26.79±2.02)mm3三组骨痂体积比较差异无统计学意义( P＝ 0.669)mm3；术，后 4周，实验组骨痂体积( 22.15±1.，66)mm3对照组骨痂体积( 29.99±1.3，2)mm3空白组骨痂体积( 29.23±1.29)mm3，对照组及空白组骨痂体积明显大于实验组( P＝0.000)。术，后第 1周，实验组骨密度(0.128±0.1，23)mg/cm3对照组骨密度(0.222± 0.121)mg/cm3，空白组骨密度( 0.217±0.103)mg/cm3，对比差异无统计学意义( P＝0.882)；术后第 2周，实验，组骨密度( 0.266±0.100)mg/cm3对照组骨密度( 0.208±0.142)mg/cm3空白组骨密度( 0.206±0.302)mg/cm3；术后第 3周，实验组骨密度( 0.218± 0.007)mg/cm3，对照组骨密度( 0.200±0.012)mg/cm3，空白组骨密度( 0.189±0.069)mg/cm3；术后第 4周，实验组骨密度( 0.225±0.009)mg/cm3对照组骨密度( 0.190±0.100)mg/cm3空白组骨密度( 0.202±0.100)mg/cm3；对照组、空白组骨密度第 2周起均低于实验组(P＜0.05，)。骨形态学发现实验组较对照组、，空白组更早形成骨痂，更早出现板层骨，更早进入塑形期。结论脑外伤合并骨折大鼠的血清对骨折愈合有明显的促进作用，为“促骨折愈合血清”应用临床治疗骨科疾患奠定理论基础。 |
| 英文摘要: |
| Objective To observe the radiological and histomorphological changes of rats with intramuscular injection fractures and brain injury("fracture?promoting serum")in rats with simple femoral fractures to determine whether the serum has accelerated frac? ture healing.Methods Twenty SD rats with fracture combined with traumatic brain injury were collected from the abdominal aortaand the serum was prepared in stages.20 healthy rats were randomly selected to extract the negative control serum，all labeled and refrigerated.The others were divided into treatment group，control group and blank group by random number table，with 32 in each group. 96 rats were middle femur fractures.Intramuscular injection of serum was used in the treatment group，intramuscular injection of negative control serum was used for the control group，and blank group was intramuscularly injected with physiological saline. Eight rats were sacrificed at the first week，2 weeks，3 weeks，and 4 weeks after operation.The healing of the three groups was com? pared by radiological analysis:X?ray irradiation，dual?energy X?ray bone density measurement and histomorphological observation. Results The X?ray results showed that the healing process of the treatment group was significantly faster than that of the controlgroup and the blank group.Measurement of osteophyte volume found:in the second week after surgery，the osteophyte volume(30.44±2.33)mm3 in the treatment group，the osteophyte volume in the control group(22.68±1.95)mm3，and the volume of the epiphysis in the blank group(21.66±2.35)mm3.The volume of the callus of the control group was significantly smaller than that of the treatment group(P＝0.000).At the third week after surgery，the volume of the callus was 26.46±1.58 mm3，and the volume of the control group was(27.29±1.66)mm3，the volume of the epiphysis in the blank group(26.79±2.02)mm3，the difference in the volume of the three groups of osteophytes was not statistically significant(P＝0.669)mm3；after 4 weeks，the volume of the epiphy? sis in the treatment group，control group and blank group was(22.15±1.66)mm3，(29.99±1.32)mm3，(29.23±1.29)mm3 seperately.The volume of the callus in the control group and the blank group was significantly larger than that in the treatment group(P＝ 0.000).At the first week after bone mineral density，the bone mineral density in the treatment group，the control group and the blank group was(0.128±0.123)mg/cm3，(0.222±0.121)mg/cm3 in，and(0.217±0.103)mg/cm3(P＝0.882)；After 2 week’s treatment， bone mineral density(0.266±0.100)mg/cm3 in the treatment group，bone mineral density(0.208±0.142)mg/cm3 in the control group， and bone mineral density in the blank group(0.206±0.302)Mg/cm3.At the third week after operation，the bone mineral density(0.218±0.007)mg/cm3 in the treatment group，bone mineral density(0.200±0.012)mg/cm3 in the control group，and bone mineral density(0.189±0.069)mg/cm3 in the blank group.After the 4th week，the bone mineral density was(0.225±0.009)mg/cm3 in the，(0.190±0.100)mg/cm3，and(0.202±0.100)mg/cm3 in the treatment group，the control group blank group.(P＜0.05).Bone morphology found that the treatment group formed osteophytes earlier than the control group and the blank group，and the lamellar bone appeared earlier，and entered the shaping stage earlier.Conclusion The serum of rats with traumatic brain injury and fracture has a signifi?cant effect on fracture healing.It lays a theoretical foundation for the clinical application of clinical treatment of orthopedic diseases. |
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