| 谈云,叶琳岚,赵珺瑶,等.五味子丙素缓解血管紧张素 Ⅱ诱导的心脏炎性反应和心肌重构[J].安徽医药,2020,24(7):1291-1295. |
| 五味子丙素缓解血管紧张素 Ⅱ诱导的心脏炎性反应和心肌重构 |
| Schisandrin C alleviates AngⅡ?induced cardiac inflammation and myocardial remodeling |
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| DOI：10.3969/j.issn.1009?6469.2020.07.005 |
| 中文关键词: 五味子 血管紧张素 Ⅱ 肿瘤坏死因子 α 白细胞介素 6 心钠素 利钠肽，脑 肌球蛋白重链 转化生长因子 β 心肌重构 炎症反应 |
| 英文关键词: Schisandra chinensis Angiotensin Ⅱ Tumor necrosis factor?alpha Interleukin?6 Atrial natriuretic factor Natriuretic peptide,brain Myosin heavy chains Transforming growth factor beta Myocardial remodeling Inflammation |
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| 中文摘要: |
| 目的探究五味子丙素对血管紧张素 Ⅱ(Ang Ⅱ)诱导小鼠心肌重构和炎性反应的影响。方法研究时间为 2018年 7月至 2019年 1月。皮下注射 Ang Ⅱ诱导 C57BL/6小鼠心肌重构。将 C57BL/6小鼠分为空白对照组、 Ang Ⅱ组、 Ang Ⅱ＋五味子丙素组(Ang Ⅱ+ShC组)皮下注射 Ang Ⅱ前一天开始给药处理， 8周后，处死小鼠，收集心脏组织。采用 ELISA法检测组织中肿瘤坏死因子 ?α(TNF?α)/白介，素?6(IL?6)的含量， RT?PCR法检测 IL?6、TNF?α、心钠肽(ANP)、脑钠肽( BNP)、肌球蛋白重链(MYHC)、转化生长因子 β(TGF?β)、胶原 IV(Collagen IV)和β?肌动蛋白(β?actin)的mRNA表达；生化试剂盒检测血液中的 CK?MB。结果与 AngⅡ组比较，五味子丙素治疗组心脏损伤被逆转，心重比降低［(6.32±0.99)‰比(4.93±0.17)‰，P＜0.05］，心功能相关基因 ANP［( 3.37±0.60)比( 1.41±0.37)倍， P＜0.05)和 BNP［( 2.51±0.75)比( 0.78±0.55)倍， P＜0.05］、促肥大基因 MYHC［( 2.57±0.74)比(1.18±0.26)倍， P＜0.05)、纤维化相关基因 TGF?β［(5.25±0.94)比( 1.78±0.57)倍， P＜0.05)和胶原 IV［(3.70±1.47)比( 1.68±0.56)倍， P＜0.05］的 mRNA表达下调，心脏组织 TNF?α/IL?6的 mRNA［分别为( 7.18±2.85)比( 2.92±0.86)倍， P＜0.05和( 12.89±5.40)比(4.29±3.43)倍， P＜0.05］和蛋白表达水平［分别为( 4.80±0.78)比(2.09±0.41)ng/mg，P＜0.05和(2.56±0.73)比( 1.23±0.41)ng/mg， P＜0.05］均显著降低。结论五味子丙素能有效缓解 Ang Ⅱ诱导的小鼠心肌重构，并伴随缓解炎性反应。 |
| 英文摘要: |
| Objective To investigate the effects of schisandrin C on alleviating myocardial remodeling and inflammation induced by angiotensin Ⅱ(AngⅡ)in mice.Methods The research period is from July 2018 to January 2019.Ang Ⅱ was injected subcutane?ously to induce cardiac remodeling in C57BL/6 mice.C57BL/6 mice were divided into blank control group，AngⅡ group and AngⅡ+schisandrin C group.The mice were subcutaneously injected with AngⅡ one day before the drug treatment was started.8 weeks later， the mice were sacrificed and cardiac tissue was collected.The contents of tumor necrosis factor?α(TNF?α)/interleukin?6(IL?6) were detected by ELISA，the mRNA expressions of IL?6，TNF?α，ANP，BNP，MYHC，TGF?β，col?4 andβ?actin were detected by RT? PCR，and CK?MB in blood was detected by biochemical kit.Results Compared with AngⅡ group，cardiac injury in the schisan? drin treatment group was reversed，the heart weight ratio decreased［(6.32±0.99‰)vs.(4.93±0.17‰)，P＜0.05］，the expressions of cardiac function related genes ANP［(3.37±0.60)vs.(1.41±0.37)times，P＜0.05)and BNP(2.51±0.75)vs. 0.78±0.55)times，P＜0.05］，hypertrophic gene MYHC［( 2.57±0.74)vs.(1.18±0.26)times，P＜0.05)and fibrosis related genesTGF?β［( 5.25±0.94)vs.(1.78±0.57)times，P＜0.05)and Collagen IV(3.70±1.47)vs. 1.68±0.56)times，P＜0.05］were decreased，and the mRNA［(7.18±2.85) vs.(2.92±0.86) times，P＜0.05 and(12.89±5.40) vs.(4.29±3.43) times，P＜0.05，respectively］ and protein expression［(4.80±0.78)vs.2.09±0.41)ng/mg，P＜0.05 and(2.56±0.73)vs.(1.23±0.41)ng/mg，P＜0.05，respectively］of TNF?a/IL?6 in cardi? ac tissue decreased significantly.Conclusion Schisandrin C can effectively alleviate the myocardial remodeling induced by AngⅡ in mice，accompanied by alleviating inflammatory response. |
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