| 胡志军,吴希静,潘晓龙,等.碳青霉烯类耐药大肠埃希菌耐药基因型检测及同源性分析[J].安徽医药,2020,24(7):1464-1467. |
| 碳青霉烯类耐药大肠埃希菌耐药基因型检测及同源性分析 |
| Detection and homology analysis of carbapenem?resistant genotypes of escherichia coli |
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| DOI：10.3969/j.issn.1009?6469.2020.07.049 |
| 中文关键词: 埃希氏菌属 序列同源性 基因， MDR 大肠埃希菌 碳青霉烯酶 基因间重复一致序列聚合酶链反应技术 |
| 英文关键词: Escherichia Sequence homology Genes,MDR Escherichia coli Carbapenemase Enterobacterial repetitive intergenic consensus polymerase chain reaction(ERIC?PCR) |
| 基金项目:铜陵市卫生局科研课题项目(卫科研〔 2014〕31号) |
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| 中文摘要: |
| 目的检测临床分离碳青霉烯类耐药大肠埃希菌的耐药基因型，并对其同源性进行分析，研究其流行情况。方法收集铜陵市人民医院 2012年 9月至 2016年 10月临床分离碳青霉烯类耐药大肠埃希菌，采用 VITEK?2 Compact全自动微生物鉴定仪进行鉴定，改良 Hodge试验检测碳青霉烯酶表型， EDTA双纸片协同试验进行金属酶初筛，聚合酶链反应( PCR)检测碳青霉烯酶基因( blaKPC、blaIMP、blaVIM、blaNDM和 blaOXA?48)并对阳性扩增产物进行基因测序；同源性分析采用肠杆菌科基因间重复一致序列聚合酶链反应技术( ERIC?PCR)。结果共收，集碳青霉烯类耐药大肠埃希菌 25株， 8株改良 Hodge试验阳性， 13株金属酶初筛试验阳性，其中 4株携带 blaNDM?1基因， 1株携带 blaIMP?4基因， 1株携带 blaKPC?2基因； ERIC?PCR检测分为 10种型别。结论碳青霉烯类耐药大肠埃希菌的耐药机制主要是产金属酶，携带 NDM?1型碳青霉烯酶大肠埃希菌需重点监测；碳青霉烯类耐药大肠埃希菌未在医院引起克隆流行。 |
| 英文摘要: |
| Objectives To detect the drug?resistant genotypes of clinically isolated carbapenem?resistant Escherichia coli，and ana? lyze their homology to study their epidemic situation.Methods From September 2012 to October 2016，clinically isolated carbapen? em?resistant Escherichia coli were collected in Tongling people’s Hospital and identified by VITEK?2 Compact automatic microbio?logical identification instrument.Modified Hodge test was performed to screen the phenotype of carbapenemase，and metalloenzymes were screened by EDTA double?disc synergistic test.Carbapenemase gene(blaKPC，blaIMP，blaVIM，blaNDM and blaOXA?48)was detected by polymerase chain reaction(PCR).The positive amplified products were sequenced and the homology analysis and typing of ERIC? PCR were carried out.Results A total of 25 strains of carbapenem?resistant Escherichia coli were collected，8 strains were positive for the modified Hodge test，and 13 strains were positive for the metalloenzyme screening test.Four of them carried blaNDM?1 gene，one strain carried blaIMP?4 gene and one strain carried blaKPC?2 gene.ERIC?PCR detection was divided into 10 types.Conclusions The main mechanism of carbapenem?resistant Escherichia coli is the production of metalloenzymes.TheNDM?1 type carbapenem?resis?tant Escherichia coli needs to be monitored.Carbapenem?resistant Escherichia coli did not cause clonal epidemics in hospitals. |
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