| 纪世彬.高效液相色谱?二极管阵列检测器法测定复合维生素 B片中 5种组分的溶出度[J].安徽医药,2020,24(8):1512-1514. |
| 高效液相色谱?二极管阵列检测器法测定复合维生素 B片中 5种组分的溶出度 |
| Determination of dissolution rate of five components in compound vitamin B tablets by HPLC?DAD |
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| DOI：10.3969/j.issn.1009?6469.2020.08.007 |
| 中文关键词: 复合维生素 B/分析 高效液相色谱 ?二极管阵列检测器(HPLC?DAD)法 回收率 溶出度 |
| 英文关键词: Vitamin B compound/analysis HPLC?DAD Retrieving rate Dissolution rate |
| 基金项目: |
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| 摘要点击次数: 2102 |
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| 中文摘要: |
| 目的建立高效液相色谱 ?二极管阵列检测器(HPLC?DAD)法同时测定复合维生素 B片中的 5种有效成分的溶出度。方法采用 Waters XBridge C18色谱柱，以 5 mmol/L庚烷磺酸钠水溶液(含 0.15%三乙胺，用冰醋酸调节 pH值至 5.0)为流动相 A，乙腈为流动相 B，流速为 1.0 mL/min，梯度洗脱，柱温 35 ℃，维生素 B1、维生素 B2、维生素 B6和烟酰胺的检测波长为 280 nm，泛酸钙的检测波长为 210 nm。结果维生素 B1、维生素 B2、烟酰胺、维生素 B6和泛酸钙分别在 9.46~47.29、7.97~39.83、39.92、199.60、1.21~6.04、3.84~19.22 μg/mL范围内线性关系良好， r均大于 0.999。5种组分的回收率在 94.3%~102.7%之间， RSD≤2.0%。结论 HPLC?DAD法同时测定复合维生素 B片中的 5种有效成分的溶出度可为复合维生素 B片的质量控制提供参考。 |
| 英文摘要: |
| Objective To establish a method for simultaneous determination of dissolution rate of five effective components in com?pound vitamin B tablets by HPLC?DAD.Methods Waters XBridge C18 was used.Mobile phase A is 5 mmol/L sodium heptanesulfo? nate solution(containing 0.15% Triethylamine，adjusted pH to 5.0 with glacial acetic acid)，Mobile phase B is acetonitrile.The flowrate was 1.0 mL/min.Column temperature was 35℃.The UV detection wavelength of vitamin B1，vitamin B2，vitamin B6 and nicotin? amide was 280 nm，and the UV detection wavelength of calcium pantothenate was 210 nm.Results The linear ranges of vitamin B1，vitamin B2，nicotinamide，vitamin B6 and calcium pantothenate were 9.46?47.29，7.97?39.83，39.92?199.60，1.21?6.04，and 3.84?19.22 μg/mL，respectively，and r was greater than 0.999.The recovery rates of the five components ranged from 94.3% to 102.7%，and RSD≤2.0%.Conclusion HPLC?DAD method for simultaneous determination of the dissolution rate of five active ingredients in compound vitamin B tablets can provide a reference for the quality control of compound vitamin B tablets. |
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