| 刘俊英,郭志玲.芍药苷通过调控硫氧还蛋白结合蛋白表达对脂多糖诱导的肾小球系膜细胞氧化应激及凋亡的影响[J].安徽医药,2021,25(4):659-663. |
| 芍药苷通过调控硫氧还蛋白结合蛋白表达对脂多糖诱导的肾小球系膜细胞氧化应激及凋亡的影响 |
| Effects of paeoniflorin on oxidative stress and apoptosis induced by lipopolysaccharide in glomerular mesangial cells by regulating TXNIP gene expression |
| |
| DOI：10.3969/j.issn.1009-6469.2021.04.006 |
| 中文关键词: 中草药 芍药属 芍药苷 硫氧还蛋白质类 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化酶 脂多糖 肾小球系膜细胞 氧化应激 凋亡 |
| 英文关键词: thione peroxidase Lipopolysaccharide Mesangial cells Oxidative stress Apoptosis |
| 基金项目: |
|
| 摘要点击次数: 1525 |
| 全文下载次数: 573 |
| 中文摘要: |
| 目的探讨芍药苷对脂多糖(LPS)诱导的肾小球系膜细胞氧化应激及凋亡的影响及其作用机制。方法研究起止时间为 2018年 1月至 2019年 7月，体外培养人肾小球系膜细胞(HMCL)用不同浓度的(5、10、20 μmol/L)芍药苷处理 LPS诱导的 HMCL细胞。采用流式细胞仪检测细胞凋亡率；应用试剂盒检测细胞中，丙二醛(MDA)量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性；实时荧光定量聚合酶链反应(qRT-PCR)与蛋白质迹法(Western blotting)分别检测硫氧还蛋白结合蛋白(Thioredoxin-interacting protein，TXNIP)表达；观察干扰 TXNIP的表达对 LPS诱导的 HMCL细胞氧化应激与凋亡的影响。结果与对照组(Con)比较， LPS组细胞凋亡率升高［(6.58±0.66)%比(28.41±2.83)%］(P<0.05)MDA含量增加［(1.26±0.13) nmol/L比(5.06±0.49)nmol/L］(P<0.05)， SOD［(26.41±2.31)U/mL比(12.46±1.21)U/mL］、 GSH-Px［(，45.61±4.13)U/mL比(8.12±0.83)U/mL］活性下降(P<0.05)TXNIP信使 RNA(mRNA)及蛋白表达升高［(1.02±0.09)vs(2.54±0.25)；(0.42±0.04)比(0.87± |
| 英文摘要: |
| Objective To investigate the effects of paeoniflorin on oxidative stress and apoptosis induced by lipopolysaccharide(LPS) in glomerular mesangial cells.Methods The research was carried out from January 2018 to July 2019. LPS was used as a stimulating factor to stimulate HMCL cells, and then LPS-induced HMCL cells were treated with different concentrations of (5, 10, 20 μmol/L) paeoniflorin. Apoptosis rate was measured by flow cytometry. The content of malondialdehyde (MDA) and superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) in cells were detected by using a kit. Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the expression of thioredoxin-interacting protein (TXNIP) in LPS-induced HMCL cells. The effect of interference with TXNIP expression on oxidative stress and apoptosis induced by LPS in HMCL cells was observed.Results Compared with the Con group, the apoptosis rate and the content of MDA were increased [(6.58±0.66)% vs (28.41±2.83)%; (1.26±0.13)nmol/L vs (5.06±0.49)nmol/L; both P<0.05], the activities of SOD [(26.41±2.31)U/mL vs (12.46±1.21)U/mL] and GSH-Px [(45.61±4.13)U/mL vs (8.12±0.83)U/mL] were decreased (P<0.05), and the expressions of TXNIP mRNA and protein were increased [(1.02±0.09) vs (2.54±0.25);(0.42±0.04) vs (0.87±0.05)](P<0.05) in the LPS group. Compared with the LPS group, the apoptosis rates ofpaeoniflorin with different concentrations were decreased [(28.41±2.83)% vs (22.16±2.17)/(16.48±1.03)/(11.25±1.12)%](P<0.05), the content of MDA was decreased [(5.06±0.49)nmol/L vs (4.12±0.41)/(2.94±0.29)/(1.68±0.17)nmol/L](P<0.05), and the activities of SOD [(12.46±1.21)U/mL vs (15.46±1.52)/(18.24±1.63)/(22.54±2.03)U/mL] and GSH-Px [(8.12±0.83)U/mL vs (17.62±1.74)/(26.14±2.63)/ (39.44±3.25)U/mL] were increased (P<0.05), but the expressions of TXNIP mRNA and protein were decreased [(2.54±0.25) vs (2.13 |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
