| 王晓阳,谢爱敏,郎少磊.血清微小 RNA-200C、微小 RNA-101、肝肠钙黏蛋白和糖类抗原 72-4联合检测对胃癌的诊断价值[J].安徽医药,2021,25(4):805-809. |
| 血清微小 RNA-200C、微小 RNA-101、肝肠钙黏蛋白和糖类抗原 72-4联合检测对胃癌的诊断价值 |
| Diagnostic value of combined detection of serum microRNA-200C, microRNA-101, liver intestine-cadherin and carbohydrate antigen 72-4 in gastric cancer |
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| DOI：10.3969/j.issn.1009-6469.2021.04.042 |
| 中文关键词: 胃肿瘤 微 RNAs 钙黏着糖蛋白类 miR-200C miR-101 肝肠钙黏蛋白 肿瘤标志物 诊断 |
| 英文关键词: Stomach neoplasms MicroRNAs Cadherins MiR-200C MiR-101 Enterohepatic cadherin Tumor marker Diagnosis |
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| 中文摘要: |
| 目的探究血清微小 RNA-200C(miR-200C)、微小 RNA-101(miR-101)、肝肠钙黏蛋白(CDH17)及糖类抗原 72-4(CA72-4)联合诊断胃癌的价值。方法选择 2017年1月至 2019年2月三门峡市中心医院收治的 150例胃癌病人及同期收治的 50例胃良性疾病病人及 50例健康体检者作为研究对象，分别纳入胃癌组、胃良性疾病组及对照组，采用实时荧光定量 -反转录聚合酶链式反应检测血清 miR-200C、miR-101水平，采用酶联免疫吸附法、电化学发光免疫分析法检测血清 CDH17及CA72-4浓度，分析各指标与胃癌临床参数的关系及其诊断价值。结果胃癌组病人血清 miR-200C［(1.10±0.22)(0.68±0.15)(0.95±0.20)］、 miR-101［1.45(0.29，2.01)0.30(0.10，0.65)、1.32(0.33，1.85)］表达水平显著低于良性组及对照组， CDH17［38.59(18.59，68.66)、88.12(33.25，152.12)、41.74(25.14，75.45)］、CA72-4［5.22(3.09，15.14)、17.15(6.71，32.12)、6.42(2.71，17.63)］水平显著高于良性组及对照组(P<0.05)。血清 miR-200C、miR-101、CDH17与胃癌病人浸润深度、淋巴结转移、脉管浸润、临床分期相关(P<0.05)； CA72-4与胃癌病人淋巴结转移相关(P<0.05)。miR-200C、miR-101、CDH17及 CA72-4诊断胃癌的曲线下面积分别为 0.899、0.904、0.871、0.793；其中 miR-101诊断曲线下面积最大。 miR-200C、miR-101、CDH17分别联合 CA72-4诊断胃癌的曲线下面积为 0.956、0.947、0.961，四种联合诊断曲线下面积最大，其曲线下面积为 0.995。结论 miR-200C、miR-101、CDH17、CA72-4对胃癌有一定诊断效能，相比于单项检测常见肿瘤标志物 CA72-4，miR-200C、miR-101、CDH17诊断价值更高，临床上可通过联合多种指标共同诊断以提高诊断效能。 |
| 英文摘要: |
| Objective To explore the value of combined detection of serum microRNA-200C (miR-200C), microRNA-101 (miR-101), liver intestine-cadherin (CDH17) and carbohydrate antigen 72-4 (CA72-4) in the diagnosis of gastric cancer.Methods A total of 150 patients with gastric cancer and 50 patients with benign gastric diseases admitted to Sanmenxia Central Hospital from January 2017 toFebruary 2019, and 50 healthy individuals were selected as study subjects. They were included into gastric cancer group, benign groupand control group, respectively. The levels of serum miR-200C and miR-101 were detected by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction. The concentrations of serum CDH17 and CA72-4 were detected by enzyme-linked immunosorbent assay and electrochemical luminescence immunoassay method. The relationship between each index and clinical parameters of gastric cancer and their diagnostic values were analyzed.Results The expression levels of serum miR-200C [(1.10±0.22), (0.68±0.15), (0.95±0.20)] and miR-101 [1.45 (0.29, 2.01), 0.30 (0.10, 0.65), 1.32 (0.33, 1.85)] in gastric cancer group were significantly lower thanthose in benign group and control group, while the levels of CDH17 [38.59 (18.59, 68.66), 88.12 (33.25, 152.12), 41.74 (25.14, 75.45)]and CA72-4 [5.22 (3.09, 15.14), 17.15 (6.71, 32.12), 6.42 (2.71, 17.63)] were significantly higher than those in benign group and control group (P<0.05). Serum miR-200C, miR-101 and CDH17 were related to invasive depth, lymph node metastasis, vascular invasion andclinical stage in patients with gastric cancer (P<0.05). CA72-4 was associated with lymph node metastasis in patients with gastric cancer (P<0.05). The areas under the curve (AUC) of miR-200C, miR-101, CDH17 and CA72-4 for the diagnosis of gastric cancer were 0.899, 0.904, 0.871 and 0.793, respectively, among which AUC of miR-101 was the greatest. AUC of miR-200C, miR-101 and CDH17 respectively combined with CA72-4 for the diagnosis of gastric cancer were 0.956, 0.947 and 0.961, respectively. AUC of the four combinationwas the greatest, which was 0.995.Conclusion The miR-200C, miR-101, CDH17 and CA72-4 are of certain diagnostic efficiency for gastric cancer. Compared with single common tumor marker CA72-4, the diagnostic values of miR-200C, miR-101 and CDH17 are higher. Clinically, combination diagnosis with multiple indexes can be applied to improve diagnostic efficiency. |
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