| 董旭,韩学昌,邢群智,等.地佐辛对坐骨神经慢性压迫性疼痛大鼠胶质细胞活性及镇痛效能的影响研究[J].安徽医药,2021,25(4):830-833. |
| 地佐辛对坐骨神经慢性压迫性疼痛大鼠胶质细胞活性及镇痛效能的影响研究 |
| Effects of dizoxine on glial cell activity and analgesic efficacy in rats with chronic compression pain of sciatic nerve |
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| DOI：10.3969/j.issn.1009-6469.2021.04.048 |
| 中文关键词: 地佐辛 坐骨神经痛 神经胶质原纤维酸性蛋白质 神经反馈 胶质细胞活性 镇痛 |
| 英文关键词: Dizoxin Sciatica Glial fibrillary acidic protein Neurofeedback Glial cell activity Analgesic |
| 基金项目: |
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| 中文摘要: |
| 目的探索地佐辛对坐骨神经慢性压迫性疼痛(chronic constriction injury，CCI)大鼠胶质细胞及镇痛效能的影响研究。方法 2018年 10月至 2020年 10月，选取雄性 SD大鼠 40只，采用随机数字表法将大鼠分为四组，每组 10只:对照组、模型组和低剂量、高剂量组。于手术后即刻至术后 7d，各组每天腹腔注射药物 1次:低剂量、高剂量组分别给予浓度为 2.5 mg/kg、10 mg/ kg的地佐辛，对照组和模型组给予等容积的 0.9%生理盐水。于给药 7d后，观察大鼠脊髓 L3～4节段胶质细胞特异性蛋白(Iba-1)和胶质原纤维酸性蛋白(GFAP)的蛋白表达水平。造模第 1、3、5、7d定大鼠病侧机械缩足反射阈(MWT)和热缩足反射潜伏期(TWL)。结果模型组大鼠脊髓中 Iba-1及 GFAP的蛋白表达水平最高，分别为(0.09±0.003)、(2.12±0.35)对照组水平最低，分别为(0.05±0.001)、(1.01±0.26)两组在 Iba-1及 GFAP表达水平上的差异有统计学意义(P<0.05)；低剂量组的，GFAP表达水平为(1.65±0.12)，低于模型组的(2.1，2±0.35)，两组在 GFAP表达水平上的差异具有统计学意义(P<0.05)；高剂量组的 GFAP表达水平为(1.36±0.25)低于模型组的(2.12±0.35)两组在 GFAP表达水平上的差异具有统计学意义(P<0.05)；在各个时间段，低剂量组、高剂量组的M，WT和 TWL均明显下降，，低剂量组的 MWT分别为(32.5±6.3、25.4±6.4、42.1±6.4、56.2±3.4)，低剂量组的 TWL(14.5±0.4、12.6±0.6、13.4±0.9、17.1±0.5)高剂量组的 MWT分别为(33.9±7.3、23.5±5.3、41.9±6.3、57.0±5.3)高剂量组的 TWL分别为(13.4±0.6、15.5±0.7、18.6±0.2、19.1±0.5)，两组在各时间段 MWT与 TWL的差异均具有统计学意义(P<0.0，5)；模型组的 MWT和 TWL分别为(31.5±5.6、29.5±5.1、21.5±4.1、2，2.5±5.3)与(16.2±3.2、11.5±3.8、9.1±2.3、8.1±1.9)其中 MWT均低于低剂量组与高剂量组，差异均具有统计学意义(P<0.05)，TWL均高于低剂量组与高剂量组，差异均具有统，计学意义(P<0.05)；对照组的 MWT和 TWL分别为(63.5±4.4、64.5±3.4、64.6±4.6、64.2±3.8)与(19.5±0.5、19.6±1.0、19.4±0.4、19.7±0.8)与模型组，低剂量组，高剂量组间的差异均具有统计学意义(P<0.05)。结论地佐辛能有效缓解 CCI模型大鼠的神经病理性痛，地疼，佐辛可抑制胶质细胞活性及迁移，达到下调 GFAP蛋白水平的效果，其镇痛机制可能与此有关。 |
| 英文摘要: |
| Objective To explore the effects of dezocine on the glial cells and analgesic efficacy of rats with chronic constriction injury (CCI) of the sciatic nerve.Methods Forty male SD rats were selected from October 2018 to October 2020, and the rats were randomly assigned into 4 groups using a random number table method, with 10 rats in each group: control group, model group, low dosegroup and high dose group. Immediately after the operation and 7d after the operation, each group was injected intraperitoneally once aday: the low-dose group and the high-dose group were given dizocine with concentrations of 2.5 mg/kg and 10 mg/kg, respectively, andthe control group and the model group were given the same volume 0.9% Physiological saline. The expression levels of glial cell-specific protein (iba-1) and glial fibrillary acidic protein (GFAP) in l3-4 segments of rat spinal cord were observed 7 days after administration.The mechanical reflex threshold (MWT) and heat reflex latency (TWL) of the sick side of the rat were determined 1, 3, 5 and 7 days after CCI modeling.Results The protein expression levels of Iba-1 and GFAP in the spinal cord of rats in the model group were the highest (0.09±0.003) and (2.12±0.35), respectively, while those in the control group were the lowest (0.05±0.001) and (1.01±0.26), respectively. The differences in the expression levels of Iba-1 and GFAP between the two groups were statistically significant (P < 0.05). The expression level of GFAP in the low-dose group was (1.65±0.12), which was lower than that in the model group (2.12±0.35), and the difference in GFAP expression between the two groups was statistically significant (P < 0.05). The expression level of GFAP in the high-dose group was (1.36±0.25), which was lower than that in the model group (2.12±0.35), and the difference in GFAP expression betweenthe two groups was statistically significant (P < 0.05). The MWT and TWL of the low-dose and high-dose groups decreased significantly at each time period. The MWT of the low-dose group was (32.5±6.3, 25.4±6.4, 42.1±6.4, 56.2±3.4), the TWL of the low-dose group was (14.5±0.4, 12.6±0.6, 13.4±0.9, 17.1±0.5), and the MWT of the high-dose group was (33.9±7.3, 23.5±5.3, 41.9±6.3, 57.0±5.3). The TWL of the high-dose group were (13.4±0.6, 15.5±0.7, 18.6±0.2, 19.1±0.5), and there were significant differences in MWT and TWLbetween the two groups at each time period (P < 0.05). MWT and TWL of the model group were (31.5±5.6, 29.5±5.1, 21.5±4.1, 22.5±5.3) and (16.2±3.2, 11.5±3.8, 9.1±2.3, 8.1±1.9), respectively. MWT of the model group was lower than that of the low-dose group and the high-dose group, the differences were statistically significant (P < 0.05), and TWL was higher than that of the low-dose group and the high-dose group, the differences were statistically significant (P < 0.05). The MWT and TWL of the control group were (63.5±4.4,64.5±3.4, 64.6±4.6, 64.2±3.8) and (19.5±0.5, 19.6±1.0, 19.4±0.4, 19.7±0.8), respectively, and the differences between the modelgroup, the low-dose group and the high-dose group were statistically significant (P <0.05).Conclusion Dizocine can effectively alleviate neuropathic pain in CCI model rats. Dizocine can inhibit the activity and migration of glial cells and achieve the effect of down-regulating the level of GFAP protein, and its analgesic mechanism may be related to this. |
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