| Objective To explore the expression and significance of tumor necrosis factor α stimulating gene-6 (TSG-6) and CXC chemokine ligand 8 (CXCL8) in different parts of keloid.Methods From March 2016 to October 2018, 25 patients with keloid underwent surgical resection combined with radiotherapy in Huaihe Hospital of Henan University were selected. The keloid tissues of infiltrating, proliferating and aging parts of each patient were collected during the operation, and 17 normal skin controls were taken frompatients with limb injury. Enzyme linked immunosorbent assay (ELISA) was used to detect the expression levels of TSG-6 protein, CXCL8 protein, apoptosis and collagen metabolism related proteins.Results In the control group, the aging part group, the hyperplasia part group, and the infiltrating part group, the expressions of TSG-6, the second mitochondria-derived activator of caspase (SMAC) , caspase-3 and IGF-1 decreased sequentially (P < 0.05), while the expression of CXCL8, apoptosis related protein B-cell lymphoma-2 (Bcl2), melanoma inhibitor of apoptosis protein (Livin), collagen metabolism related protein transforming growth factor-β 1(transforming growth factor β 1,TGF-β1), type I collagen (CoL-I) and matrix metalloproteinase 2 (MMP-2) increased sequentially (P<0.05) . In the control group, infiltrating part group, proliferating part group and aging part group, the TGS-6 protein expression level was (1.81±0.19),(0.34±0.07), (0.64±0.12) and (1.37±0.14) ng/mL, respectively; the CXCL8 expression level was (37.54±5.61), (248.65±40.62), (174.01±26.25) and (66.43±10.23) ng/mL, respectively; Bcl-2 protein expression level was (61.87±2.12), (167.21±6.34), (114.45±3.26), (81.64±3.41) ng/mL, respectively; SmaC protein expression level was (618.42±47.85), (176.55±19.64), (348.71±31.58), (424.54±36.13) ng/mL,respectively; Caspase-3 protein expression level was (17.67±2.64), (5.48±1.74), (8.87±1.54), (15.25±2.67) ng/mL, respectively; Livin protein expression level was (1.25±0.28), (3.94±0.35), (2.26±0.31), (1.51±0.24) ng/mL, respectively; p53 protein expression level was (2.19±0.28), (0.64±0.08), (1.58±0.11), (1.81±0.17) ng/mL, respectively; TGF-β1 protein expression level was (275.54±76.16), (421.57±56.01), (361.55±52.15), (324.16±74.72) ng/mL, respectively; Col-1 protein expression level was (0.98±0.05), (3.80±0.54), (2.51±0.61), (1.43± 0.16) ng/mL, respectively.; MMP-2 protein expression level was (2.47±0.26), (25.39±6.41), (19.12±5.45), (5.17±1.42) ng/mL, respectively; IGF-1 protein expression level was (251.05±77.25), (81.51±14.50), (130.48±21.52), (234.49±82.42) ng/mL, respectively.. Pearsoncorrelation analysis showed that the expression of TGS-6 protein in keloid patients was negatively correlated with Bcl-2, Livin, TGF-β 1, COL-1 and MMP-2(P < 0.05), and was positively correlated with SMAC, Caspase-3, p53 and IGF-1(P < 0.05); The expression of CXCL8 was positively correlated with Bcl-2, livin and TGF-β 1, COL-1 and MMP-2(P < 0.05), but was negatively correlated with SMAC, Caspase-3, p53 and IGF-1(P < 0.05).Conclusion TSG-6 and CXCL8 are expressed differently in different parts of keloid, which may be involved in the occurrence and development of keloid by regulating cell proliferation, apoptosis and collagen synthesis and metabolism. |
