| 张跃,孙彦申.微小 RNA-744-5p靶向整合素连接激酶对膀胱癌细胞增殖、迁移、侵袭的影响[J].安徽医药,2021,25(8):1622-1628. |
| 微小 RNA-744-5p靶向整合素连接激酶对膀胱癌细胞增殖、迁移、侵袭的影响 |
| Effect of miR-744-5p on proliferation, migration and invasion of bladder cancer cells and its mechanism by targeting ILK |
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| DOI：10.3969/j.issn.1009-6469.2021.08.035 |
| 中文关键词: 膀胱肿瘤 微小 RNA-744-5p 整合素连接激酶 细胞增殖 迁移 侵袭 |
| 英文关键词: Urinary bladder neoplasms MiR-744-5p Integrin-linked kinase Cell proliferation Migration Invasion |
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| 中文摘要: |
| 目的探讨微小 RNA-744-5p(miR-744-5p)对膀胱癌细胞增殖、迁移和侵袭的影响及可能机制。方法选取 2016年 2月至 2018年 11月枣庄矿业集团枣庄医院接收的经病理确诊的 56例膀胱癌病人病理组织切片，采用实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测 56例膀胱癌组织和对应癌旁组织中 miR-744-5p表达水平。分别转染 miR-744-5p模拟物( mimics)、 miR-744-5p抑制剂或共转染 miR-744-5p mimics与整合素连接激酶( ILK)过表达载体至膀胱癌 BIU-87、T739细胞， MTT检测细胞增殖， Transwell检测细胞迁移和侵袭，蛋白质印迹法( Western Blotting)检测过细胞中细胞周期蛋白( Cyclin D1)、 P21、基质金属蛋白酶( MMP-2)和钙黏附蛋白 E(E-cadherin)蛋白表达。双荧光素酶报告基因实验验证 miR-744-5p与 ILK调控关系。结果与癌旁组织相比，膀胱癌组织中 miR-744-5p表达水平显著降低［(0.98±0.10)比( 0.25±0.02)，P<0.05］。过表达 miR-744-5p后， BIU-87和 T739细胞 OD值、迁移数和侵袭数及 Cyclin D1和 MMP-2蛋白表达降低( P<0.05)，而 P21和 E-cadherin蛋白表达升高( P<0.05)。抑制 miR-744-5p后， BIU-87和 T739细胞 OD值、迁移数和侵袭数升高( P<0.05)。 miR-744-5p靶向结合并负调控 ILK，过表达 ILK逆转过表达 miR-744-5p对 BIU-87和 T739细胞增殖、迁移和侵袭的影响。结论膀胱癌组织中 miR-744-5p呈低表达，过表达 miR-744-5p可阻碍膀胱癌细胞增殖、迁移和侵袭，而抑制 miR-744-5p则起相反的作用，其可能通过靶向负调控 ILK表达发挥作用。 |
| 英文摘要: |
| Objective To investigate the effect of miR-744-5p on proliferation, migration and invasion of bladder cancer BIU-87 cells and its mechanism.Methods A total of 56 pathologically confirmed patients with bladder cancer in Zaozhuang Mining Group Zaozhuang Hospital from February 2016 to November 2018 were selected. RT-qPCR was used to detect the expression of miR-744-5p in 56 bladder cancer tissues and corresponding adjacent tissues. After miR-744-5p mimics or miR-744-5p inhibitor was transfected into bladder cancer BIU-87 and T739 cells, or miR-744-5p mimics and integrin-linked kinase (ILK) overexpression vectors were co-transfected into bladder cancer BIU-87 and T739 cells, and then MTT was used to detect the proliferation. Transwell was used to detect themigration and invasion, and Western Blot was used to detect the protein levels of Cyclin D1, P21, MMP-2 and E-cadherin. The dual luciferase reporter gene assay validated the relationship between miR-744-5p and ILK.Results The expression level of miR-744-5p in bladder cancer tissues was significantly lower than that in adjacent tissues [(0.98±0.10) vs. (0.25±0.02), P<0.05]. After overexpression of miR-744-5p, the OD value, the number of migration and invasion and the protein levels of Cyclin D1 and MMP-2 protein in BIU-87 and T739 cells were decreased (P<0.05), but the protein levels of P21 and E-cadherin were increased (P<0.05). After inhibiting miR744-5p, the OD value, the number of migration and invasion of BIU-87 and T739 cells were increased (P<0.05). miR-744-5p targeted and negatively regulated ILK. Overexpression of ILK reversed the effects of overexpression of miR-744-5p on the proliferation, migration and invasion of BIU-87 and T739 cells.Conclusion The expression of miR-744-5p in bladder cancer tissues is low. Overexpression of miR-744-5p can hinder the proliferation, migration and invasion of bladder cancer cells, but inhibition of miR-744-5p has the opposite effect, which may play a role by targeting and negatively regulating expression of ILK . |
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