| 李路鹏,李晓光,刘棚越,等.微小 RNA-202和转录因子性别决定区 Y框蛋白 9在膀胱癌组织中表达与临床病理特征及预后的关系[J].安徽医药,2021,25(10):2008-2012. |
| 微小 RNA-202和转录因子性别决定区 Y框蛋白 9在膀胱癌组织中表达与临床病理特征及预后的关系 |
| The relationship between the expression of microRNA-202 (miR-202) and transcription factor sex-determining region Y-box protein 9 (Sox9) and clinicopathological features and prognosis in bladder cancer |
| |
| DOI：10.3969/j.issn.1009-6469.2021.10.023 |
| 中文关键词: 膀胱肿瘤 微小 RNA-202 性别决定区 Y框蛋白 9 相关性 预后 |
| 英文关键词: Urinary bladder neoplasms miR-202 Sex-determining region Y-box protein 9 Correlation Prognosis |
| 基金项目:河南大学科研基金项目( 2012YBZR019) |
|
| 摘要点击次数: 1157 |
| 全文下载次数: 361 |
| 中文摘要: |
| 目的探讨微小 RNA 202(miR-202)和转录因子性别决定区 Y框蛋白 9(Sox9)在膀胱癌中的表达及与临床预后的关系。方法选取 2012年 6月至 2013年 12月河南大学第一附属医院肿瘤科收治的 120例行切除术的膀胱癌病人作为研究对象，收集癌组织( n=120)及癌旁正常组织( n=120)采用免疫组化检测组织中 Sox9蛋白表达，采用荧光定量 PCR(qRT-PCR)检测癌组织及癌旁组织中 miR-202和 Sox9表达量，观察，两组组织中 miR-202、Sox9表达水平、与临床病理参数关系、二者相关性及与病人预后关系。结果膀胱癌组织中 Sox9阳性表达率为 70.83%，癌旁组织为 31.67%，差异有统计学意义( P<0.05)；膀胱癌组织中 miR-202水平低于癌旁组织( P<0.05)，Sox9水平高于癌旁组织( P<0.05)； miR-202、Sox9表达与年龄、性别、肌层浸润无关( P>0.05)，与病理分级及淋巴结转移相关(P<0.05)；膀胱癌组织中 miR-202和 Sox9表达呈负相关性( r=?0.835，P=0.000)；膀胱癌组织中 miR-202高表达病人中位生存时间为( 48.61±1.82)个月，高于低表达组的(37.99±2.47)个月( P<0.05)miR-202高表达病人预后 5年生存率为 52.94%，高于低表达组的 30.77%(P<0.05)； Sox9高表达病人中位生存时间为( 39.78±2.60)，个月，低于低表达组的( 49.62±1.90)个月( P<0.05)Sox9高表达病人预后 5年生存率为 38.18%，低于低表达组的 60.00%(P<0.05)； TNM分期、淋巴结转移、 Sox9是影响病人预后的独，立危险因素， miR-202是影响膀胱癌病人预后的保护因素。结论膀胱癌组织中miR-202低表达， Sox9高表达，二者表达呈正相关，与病人病理分级及淋巴结转移相关， miR-202低表达与 Sox9高表达生存期短，预后差，可能作为膀胱癌预后评估的潜在标记物。 |
| 英文摘要: |
| Objective To investigate the expressions of microRNA-202 (miR-202) and transcription factor sex-determining region Y-box protein 9 (Sox9) in bladder cancer and their relationships with clinical prognosis.Methods A total of 120 patients with bladdercancer who underwent resection in Oncology Department of First Affiliated Hospital of Henan University from June 2012 to December2013 were selected as the study subjects, cancer tissues (n = 120) and normal adjacent tissues (n = 120) were collected, immunohistochemistry was used to detect the expression of Sox9 protein in tissues, quantitative fluorescence PCR (qRT-PCR) was used to detect the expressions of miR-202 and SOX-9 in cancer tissues and adjacent tissues. The expressions of miR-202 and Sox9 in the tissues, their relationships with clinicopathological parameters, the correlation between them, and their relationships with prognosis of the patients inthe two groups were observed.Results The positive expression rate of Sox9 in bladder cancer tissues was 70.83%, and that in adjacenttissues was 31.67%, and the difference was statistically significant (P<0.05); the level of miR-202 in bladder cancer tissues was lower than that in adjacent tissues (P < 0.05), and the level of Sox9 was higher than that in adjacent tissues (P < 0.05); the expressions of miR202 and Sox9 were not correlated with age, sex and muscle invasion (P > 0.05), but were correlated with pathological grade and lymph node metastasis (P < 0.05); there was a negative correlation between the expressions of miR-202 and SOX-9 in bladder cancer (r=? 0.835, P=0.000); the median survival time of patients with miR-202 high expression in bladder cancer tissue was (48.61±1.82) months,which was higher than (37.99±2.47) months of patients with low expression (P < 0.05), the 5-year survival rate of patients with miR-202 high expression was 52.94%, which was higher than 30.77% of patients with miR-202 low expression (P < 0.05); the median survivaltime of patients with Sox9 high expression was (39.78±2.60) months, which was lower than (49.62 ±1.90) months of patients with Sox9low expression (P < 0.05), the 5-year survival rate of patients with Sox9 high expression was 38.18%, which was lower than 60.00% ofpatients with Sox9 low expression (P < 0.05);TNM staging, lymph node metastasis, and Sox9 were independent risk factors for the prognosis of patients. miR-202 was a protective factor affecting the prognosis of patients with bladder cancer.Conclusions The expression of miR-202 is low and Sox9 is high in bladder cancer tissues, and they are positively correlated and related to pathological grade andlymph node metastasis. The low expression of miR-202 and high expression of Sox9 correlate with short survival time and poor prognosis, which may be potential markers for prognosis evaluation of bladder cancer. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
