| 余自淑,田莉,陆静,等.乏氧环境对子宫肌瘤细胞增殖及自噬相关蛋白表达的影响[J].安徽医药,2021,25(10):2044-2048. |
| 乏氧环境对子宫肌瘤细胞增殖及自噬相关蛋白表达的影响 |
| Effects of hypoxia on proliferation and autophagy related protein expression of uterine leiomyoma cells |
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| DOI：10.3969/j.issn.1009-6469.2021.10.031 |
| 中文关键词: 肿瘤乏氧 子宫肿瘤 平滑肌瘤 乏氧环境 细胞增殖 自噬 |
| 英文关键词: Tumor hypoxia Uterine neoplasms Leiomyoma Hypoxia environment Cell proliferation Autophagy |
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| 中文摘要: |
| 目的探究乏氧环境对子宫肌瘤细胞增殖和自噬相关蛋白表达的影响。方法选取成都市第三人民医院 2017年 11月至 2018年 11月行子宫肌瘤手术治疗的 20例病人的子宫肌瘤组织样本(观察组)及周围正常组织(对照组)进行乏氧( 1%氧)培养，培养 48 h检测两组细胞增殖及自噬相关蛋白的表达差异。结果培养 12 h开始至 48 h，两组细胞增值率明显下降，并且观察组下降更加显著( P<0.05)。随着缺氧时间增长，两组凋亡细胞比例均显著增加( P<0.05)且观察组细胞凋亡比例更高于对照组( P<0.05)。培养 0h，观察组子宫肌瘤细胞孕激素受体 A/B(PR-A/B)(0.436±0.027)(/ 0.45，9±0.033)、孕激素受体 M(PRM)(0.121±0.013)、缺氧诱导因子 1α(HIF-1α)(0.541±0.032)及孔蛋白( porin蛋白)(3.261±0.857)表达水平明显低于对照组 PRA/B(0.225±0.022，0.245±0.029)、 PR-M(0.084±0.009)、 HIF-1α(0.233±0.070)及 porin蛋白( 0.205±0.027)表达水平( P<0.05)。培养 48 h后，观察组 PR-A/B、PR-M、HIF-1α及 porin蛋白水平显著降低( P<0.05)，而对照组各蛋白水平无明显变化( P>0.05)。培养 0h，观察组酵母自噬相关基因( Beclin-1)(0.77±0.08)、选择性自噬接头蛋白( P62)(0.44±0.08)及自噬相关蛋白 Ⅱ型微管相关蛋白轻链 3(LC3-Ⅱ)(0.55±0.13)显著低于对照组 Beclin-1(1.25±0.12)、 P62(0.95±0.15)及 LC3-Ⅱ(0.81±0.09)表达水平( P<0.05)，培养 48 h后，观察组各蛋白水平明显升高( P<0.05)，而对照组无明显变化( P>0.05)。结论子宫肌瘤细胞增殖及自噬相关蛋白存在明显异常，乏氧环境促进了子宫肌瘤细胞的凋亡和自噬作用。 |
| 英文摘要: |
| Objective To explore the effect of hypoxic environment on the proliferation and autophagy related protein expression of uterine leiomyoma cells.Methods The uterine myoma tissue samples (observation group) and normal tissue (control group) of 20 patients who underwent hysteromyoma surgery in Chengdu Third People's Hospital from November 2017 to November 2018 were selectedfor hypoxia (1%O2) culture. The expression of proliferation and autophagy related protein in the two groups were detected at 48 h.Re? sults From 12 h to 48 h, the cell proliferation rate decreased significantly in the two groups, and the decrease in the observation groupwas more significant (P<0.05). With the increase of hypoxia time, the proportion of apoptotic cells in both groups increased significantly (P<0.05), and the percentage of apoptotic cells in the observation group was higher than that in the control group (P<0.05). The expression of PR-A/B (0.436±0.027, 0.459±0.033), PR-M (0.121±0.013), HIF-1α (0.541±0.032) and porin protein (3.261±0.857) in uterine leiomyoma cells in the observation group was significantly lower than PR-A/B (0.225±0.022, 0.245±0.029), PR-M (0.084±0.009), HIF1α (0.233±0.070) and porin protein (0.205±0.027) in the control group at 0 h (P<0.05). After culture for 48 h, the levels of PR-A/B, PRM, HIF-1α and porin protein in the observation group were significantly decreased (P<0.05), but the control group had no significant change in the protein levels (P>0.05). After culture for 0 h, the levels of Beclin-1 (0.77±0.08), P62 (0.44±0.08) and light chain of microtubule-associated protein type Ⅱ (LC3-Ⅱ) (0.55±0.13) in the observation group were significantly lower than Beclin-1 (1.25±0.12), P62 (0.95±0.15) and LC3-Ⅱ (0.81±0.09) in the control group (P<0.05). After 48 hours of culture, the levels of each protein in the observation group were significantly increased (P<0.05), but there was no significant change in the observation group (P>0.05).Conclusion The proliferation and autophagy related protein of uterine leiomyoma cells are obviously abnormal. Hypoxia environment promotes theapoptosis and autophagy of uterine leiomyoma cells. |
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