| 赵荣荣,张倩.胃癌血清标志物与人表皮生长因子受体 2、Ki-67表达及临床病理特征的关系[J].安徽医药,2021,25(12):2449-2453. |
| 胃癌血清标志物与人表皮生长因子受体 2、Ki-67表达及临床病理特征的关系 |
| Relationship between the serum expression of tumor markers and the protein expression of HER-2 and Ki-67 in gastric cancer and their clinicopathological characteristics |
| |
| DOI：10.3969/j.issn.1009-6469.2021.12.027 |
| 中文关键词: 胃肿瘤 受体，表皮生长因子 Ki-67蛋白 病理状态，体征和症状 癌胚抗原 糖类抗原 125 糖类抗原 19-9 |
| 英文关键词: Stomach neoplasms Receptor, epidermal growth factor Ki-67 P Pathological conditions, signs and symptoms CEA CA12-5 CA19-9 |
| 基金项目:2018年度“科大新医学”联合基金项目( WK9110000026) |
|
| 摘要点击次数: 1101 |
| 全文下载次数: 422 |
| 中文摘要: |
| 目的探讨胃癌病人手术前血清标志物癌胚抗原、糖类抗原 125(CA125)、糖类抗原 19-9(CA19-9)表达与人表皮生长因子受体 2(HER-2)和 Ki-67蛋白表达水平相关性以及与临床病理特征之间的关系。方法首先收取 132例自 2017年 10月至 2018年 12月来安徽医科大学第一附属医院高新院区住院治疗的胃癌手术病人的术前血液标本和肿瘤组织标本，然后用免疫法检测胃癌病人术前血清标志物癌胚抗原， CA125，CA19-9的表达情况，用免疫组织化学 Elivision法检测同一病人术后肿瘤组织 HER-2和 Ki-67蛋白表达情况，最后分析标志物癌胚抗原， CA125，CA19-9与 HER-2，Ki-67的相关性，以及与临床病理特征的相关性。结果 132例胃癌病人血液中发现超出正常值的癌胚抗原为 46例( 34.8%)CA125为 20例( 15.2%)CA19-9为 14例(10.6%)免疫组织化学结果发现 HER-2表达( 0)为 49例( 37.2%)，(+)为 46例( 34.8%)，(2+)为 21例( 15.9%)(3+)为 16例，(12.1%)胚抗原超出正常值的阳性表达与 HER-2表达无相关性(Z=.1.356，P=0.175)125和 CA19-9的阳达与 HER-2表达Z=.2.183，P=0.029；Z=.2.379，P=0.017)； Ki-67表达阴性 2例( 1.5%)，弱阳例( 0.8%)，中度阳性 63例( 47.7%)，阳性 66例( 50%)，HER-2表达与 Ki-67表达呈负相关( rs=.0.274，P=0.007)；同时癌胚抗原阳性与胃癌病人年龄、肿瘤大小以及肿瘤分期有关( P=0.016，P=0.046和 P=0.030)，CA125与脉管侵犯有关( P=0.026)。结论术前血清 CA125、CA19-9与术后 HER-2蛋白在胃癌中表达有关， HER-2表达与 Ki-67表达呈负相关。相关血清癌胚抗原阳性与胃癌病人年龄、肿瘤大小、肿瘤分期相关， CA125与脉管侵犯有关。 |
| 英文摘要: |
| Objective To investigate the relationship between the serum related markers carcinoembryonic antigen (CEA), carbohy.drate antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9) and the human epidermal growth factor receptor-2 (HER-2) and Ki-67 and their clinicopathological characteristics in patients of gastric cancer.Methods Firstly, we collected pre-operative blood and tumortissue specimens of 132 cases of gastric cancer undergoing surgery in Gaoxi Branch of The First Affiliated Hospital of Anhui MedicalUniversity from October 2017 to December 2018, then detected the expression of serological biomarkers CEA, CA125, CA19-9 before surgery using electrochemiluminescence immunoassay and postoperative tumor tissue HER-2, Ki-67 protein expressions for the samepatients using immunohistochemical Elivision method. Finally, we analyzed the correlation of serological markers with HER-2, Ki-67 and their correlation with clinicopathological characteristics.Results The number of gastric cancer patients in whom levels of CEA, CA125, and CA19-9 were above the normal value was 46 cases (34.8%), 20 cases (15.2), and 14 cases (10.6%), respectively. Immuno.histochemical results found that the expression of HER-2 was (0) in 49 cases (37.2%), (+) in 46 cases (34.8%), (2+) in 21 cases(15.9%), and (3+) in 16 cases (12.1%). There was no correlation between the positive expression of CEA and HER-2 expression (Z=1.356, P = 0.175), while the positive expressions of CA125 and CA19-9 were correlated with the expression of HER-2(Z=.2.183, P=0.029 and Z=.2.379, P=0.017). Ki-67 expression was negative in 2 cases (1.5%), weakly positive in 1 case (0.8%), moderately positivein 63 cases (47.7%), and strongly positive in 66 cases (50%). HER-2 expression was negatively correlated with Ki-67 expression (rs=. 0.274, P=0.007). Meanwhile, CEA positivity was associated with age, tumor size and tumor staging of gastric cancer patients (P=0.016, P=0.046 and P=0.030), and CA125 was associated with vascular invasion (P=0.026).Conclusions Detected expressions of preopera. tive serum CA125 and CA19-9 were correlated with postoperative HER-2 protein expression in gastric cancer. HER-2 expression was negatively correlated with Ki-67 expression. Serum CEA positivity was correlated with age, tumor size and tumor staging of patientswith gastric cancer, and CA125 was associated with vascular invasion. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
