| 秦铁城,杜润爽,宋雪飞.下调 RNA解螺旋酶 DDX5表达对胶质瘤 SHG44细胞周期和凋亡的影响及其机制[J].安徽医药,2021,25(12):2475-2478. |
| 下调 RNA解螺旋酶 DDX5表达对胶质瘤 SHG44细胞周期和凋亡的影响及其机制 |
| Effect of down-regulation the expression of RNA helicase DDX5 on cell cycle and apoptosis of glioma SHG44 and its mechanism |
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| DOI：10.3969/j.issn.1009-6469.2021.12.033 |
| 中文关键词: 神经胶质瘤 RNA解螺旋酶类 细胞凋亡 细胞增殖 侵袭 迁移 Wnt/β-连环素信号通路 |
| 英文关键词: Glioma RNA helicases Apoptosis Cell proliferation Invasion Migration Wnt/β-catenin signaling pathway |
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| 中文摘要: |
| 目的探讨下调 RNA解螺旋酶 DDX5表达对胶质瘤 SHG44细胞周期和凋亡的影响及其机制。方法 2018年 3月至 2019年 4月，将体外培养的 SHG44细胞分为小干扰 RNA(siRNA)-阴性对照( NC)组(转染 NC siRNA)和 siRNA-DDX5组(转染靶向 DDX5的 siRNA)，采用实时荧光定量聚合酶链反应( PCR)检测各组细胞中 DDX5 mRNA的表达水平，流式细胞仪分别检测各组细胞周期分布和凋亡率，蛋白质印迹法检测细胞中 DDX5、β-连环素、 c-Myc、细胞周期蛋白 D1(cyclin D1)和存活蛋白的表达水平。结果与 siRNA-NC组比较， siRNA-DDX5组细胞中 DDX5 mRNA［(0.25±0.02)(0.98±0.06)］和 DDX5［(0.23±0.03)比 |
| 英文摘要: |
| Objective To investigate the effect of down-regulated RNA helicase DDX5 expression on cell cycle and apoptosis of gli. oma SHG44 cells and its mechanism.Methods SHG44 cells were cultured and assigned into two groups: small interference RNA (siR. NA)-negative control (NC) group (transfected NC siRNA) and siRNA-DDX5 group (transfected siRNA targeting DDX5) from March2018 to April 2019. The expression of DDX5 mRNA in each group was detected by real-time fluorescent quantitative polymerase chainreaction (PCR), the cell cycle distribution and apoptosis rate were detected by flow cytometry, and the expression levels of DDX5, β-catenin, c-Myc, cyclin D1 and Survivin proteins were measured by Western blotting.Results Compared with the siRNA-NC group, the expression levels of DDX5 mRNA [(0.25±0.02) vs. (0.98±0.06)], DDX5 [(0.23±0.03) vs. (0.68±0.05)], β-catenin [(0.25±0.02) vs. (0.80± 0.06)], c-Myc [(0.37±0.03) vs. (0.85±0.06)], cyclin D1 [(0.30±0.02) vs. (0.89±0.06)] and Survivin [(0.42±0.03) vs. (0.83±0.0)] proteins in the siRNA-DDX5 group were significantly decreased, the percentage of cells in the G0/G1 phase was significantly reduced [(56.15± 2.28)% vs. (65.76±3.22)%], while the percentage in the S phase [(37.26±2.03)% vs. (26.95±1.16)%] and the apoptotic rate [(18.95± 2.23)% vs. (7.02±1.26)%] were significantly increased (P<0.05).Conclusion Down-regulation of DDX5 expression can induce gliomaSHG44 cell cycle arrest in S phase and promote apoptosis, and its mechanism may be related to inhibiting the activation of Wnt/β-catenin signaling pathway. |
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