| 周自广.人参皂苷Rh2 调控微小RNA-133a-3p 对瘢痕疙瘩成纤维细胞凋亡的影响[J].安徽医药,2022,26(5):873-876. |
| 人参皂苷Rh2 调控微小RNA-133a-3p 对瘢痕疙瘩成纤维细胞凋亡的影响 |
| Effect of ginsenoside Rh2 on the apoptosis of keloid fibroblasts by regulating miR-133a-3p |
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| DOI：10.3969/j.issn.1009-6469.2022.05.006 |
| 中文关键词: 人参皂苷类 瘢痕疙瘩 成纤维细胞 微小RNA-133a-3p Wnt/β-连环素信号通路 凋亡 |
| 英文关键词: Ginsenosides Keloid Fibroblasts MiR-133a-3p Wnt/β-catenin signaling pathway Apoptosis |
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| 中文摘要: |
| 目的探讨人参皂苷Rh2调控微小RNA(miR)-133a-3p对瘢痕疙瘩成纤维细胞(KF)凋亡的影响。方法用浓度为50、100、200 mg/L的人参皂苷Rh2培养KF，未给人参皂苷Rh2的KF记为对照(NC)组；将miR-NC、miR-133a-3p分别转染至KF中记为miR-NC组、miR-133a-3p组；将anti-miR-NC、anti-miR-133a-3p分别转染至KF再用200 mg/L的人参皂苷Rh2处理记为anti-miR-NC+人参皂苷Rh2组、anti-miR-133a-3p+人参皂苷Rh2组。流式细胞术检测细胞凋亡；实时荧光定量PCR(RT-qPCR)检测miR-133a-3p表达水平；蛋白质印迹法检测裂解的半胱氨酸天冬氨酸蛋白酶-3(Cleaved caspase-3)、β-连环素(β-catenin)表达。结果与对照组相比，50、100、200 mg/L 人参皂苷Rh2 处理的KF 中细胞凋亡率升高［(9.08±0.91)%、(16.28±1.63)%、(23.18±2.32)%比(4.29±0.43)%］，miR-133a-3p表达水平升高，β-catenin表达水平降低(P<0.05)。过表达miR-133a-3p，Cleavedcaspase-3表达水平升高，细胞凋亡率升高(P<0.05)。低表达miR-133a-3p部分逆转了人参皂苷Rh2对KF凋亡和β-catenin的影响。结论人参皂苷Rh2可促进瘢痕疙瘩成纤维细胞凋亡，其机制可能与miR-133a-3p和Wnt/β-catenin信号通路有关。 |
| 英文摘要: |
| Objective To investigate the effect of ginsenoside Rh2 on the apoptosis of keloid fibroblasts (KF) by regulating miR-133a-3p.Methods KF was cultured with ginsenoside Rh2 at different concentrations of 50, 100, 200 mg/L, and KF without ginsen-oside Rh2 was recorded as control (NC) group; miR-NC and miR-133a-3p were transfected to KF, which was recorded as miR-NC group and miR-133a-3p group; anti-miR-NC and anti-miR-133a-3p were transfected into KF and treated with 200 mg/L ginsenoside Rh2 as anti- miR-NC+ginsenoside Rh2 group, anti-miR-133a-3p+ginsenoside Rh2 group. Apoptosis was detected by flow cytometry;the expression of miR-133a-3p was detected by real-time quantitative PCR (RT-qPCR); the expression of cleaved cysteine-containing aspartate-specific proteases-3 (Cleaved caspase-3) and β-catenin was detected by Western blot.Results Compared with control group,the apoptosis rate of KF treated with 50, 100, 200 mg/L of ginsenoside Rh2 was significantly increased [(9.08±0.91)%, (16.28±1.63)%,(23.18±2.32)% vs. (4.29±0.43)%], the expression of miR-133a-3p was significantly increased, and the expression of β-catenin was sig-nificantly decreased (P<0.05). Overexpression of miR-133a-3p, the expression of Cleaved caspase-3 was significantly increased, and the apoptosis rate was significantly increased (P<0.05). Low expression of miR-133a-3p partially reversed the effect of ginsenoside Rh2 on KF apoptosis and β-catenin.Conclusion Ginsenoside Rh2 promotes apoptosis of keloid fibroblasts, and its mechanism may be re-lated to miR-133a-3p and Wnt/β-catenin signaling pathways. |
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