| 杨雪,冯志星,马海滨,等.微小RNA-107 调控内质网应激对喉癌Hep-2 细胞癌增殖及凋亡的影响[J].安徽医药,2022,26(5):904-907. |
| 微小RNA-107 调控内质网应激对喉癌Hep-2 细胞癌增殖及凋亡的影响 |
| Effect of miR-107 on the proliferation and apoptosis of laryngeal carcinoma Hep-2 cells by regulating endoplasmic reticulum stress |
| |
| DOI：10.3969/j.issn.1009-6469.2022.05.013 |
| 中文关键词: 微小RNA-107 内质网应激 喉癌Hep-2细胞 增殖 凋亡 |
| 英文关键词: MiR-107 Endoplasmic reticulum stress Laryngeal carcinoma Hep-2 cells Proliferation Apoptosis |
| 基金项目:河北省医学科学研究项目(20200485) |
|
| 摘要点击次数: 1032 |
| 全文下载次数: 340 |
| 中文摘要: |
| 目的探讨微小RNA(miR-107)调控内质网应激对喉癌Hep-2细胞增殖和凋亡的影响。方法将miR-107类似物和阴性对照片段转染至对应组细胞中，以未转染组作为空白组。采用荧光定量PCR(RT-PCR)检测各组Hep-2细胞中miR-107相对表达量，用蛋白质印迹法(Western blotting)检测各组Hep-2 细胞中的内质网应激有关蛋白即蛋白激酶R 样内质网激酶(PERK)及活化转录因子4(ATF4)蛋白的表达，采用四甲基偶氮唑盐(MTT)比色法测定Hep-2细胞的增殖能力，用流式细胞术检测细胞凋亡情况。结果三组转染细胞miR-107相对表达量差异有统计学意义(χ2=11.50，P<0.05)。其中mimics组miR-107的相对表达量为(15.32±1.94)，与mimics组相比较，NG组、空白组miR-107相对表达量均降低(P<0.05)。三组转染细胞的PERK和ATF4蛋白表达水平均差异有统计学意义(F=70.35、13.77，P<0.05)。其中mimics组PERK 和ATF4蛋白的相对表达量为(0.53±0.07)和(0.75±0.06)，与mimics组相比较，NG组和空白组的PERK和ATF4蛋白相对表达量均降低(P<0.05)。MTT分析结果显示，三组细胞的增殖能力差异有统计学意义(F=21.44、28.38、59.14，P<0.05)。其中mimics组Hep-2细胞增殖能力在12、24、36 h时分别为(0.15±0.01)、(0.33±0.03)和(0.47±0.04)，与mimics组比较，在12、24、36 h后NG组、空白组Hep-2细胞增殖能力均有显著升高(P<0.05)。三组转染细胞的细胞凋亡率比较差异有统计学意义(F=102.32，P<0.05)。其中mimics组Hep-2细胞凋亡率为(10.18±0.93)，与mimics组相比较，NG组和空白组的细胞凋亡率均降低(P<0.05)。结论上调miR-107的表达能够抑制Hep-2细胞的增殖，促进Hep-2细胞凋亡，内质网应激可能在其中有重要作用。 |
| 英文摘要: |
| Objective To investigate the effect of miR-107 on the proliferation and apoptosis of laryngeal carcinoma Hep-2 cells by regulating endoplasmic reticulum stress .Methods The miR-107 analog and negative control fragment were transfected into the corre-sponding group of cells, and the untransfected group was used as the blank group. Fluorescence quantitative PCR (RT-PCR) was used to detect the relative expression of miR-107 in Hep-2 cells in each group, and Western blot (WB) was used to detect the expression of endoplasmic reticulum stress proteins including protein kinase R-like ER kinase (PERK) and Activating Transcription Factor 4 (ATF4) protein in Hep-2 cells in each group. Tetramethylazolyzole (MTT) Chromatography was used to determine the proliferation ability of Hep-2 cells, and flow cytometry was used to detect cell apoptosis.Results The relative expression of miR-107 in the three groups of transfected cells was significantly different (χ2=11.50, P<0.05). The relative expression of miR-107 in the mimics group was (15.32±1.94). Compared with the mimics group, the relative expression of miR-107 in the NG group and the blank group was decreased (P<0.05). The differences in the expression levels of PERK and ATF4 proteins of the three groups of transfected cells were statistically sig-nificant (F=70.35,13.77,P<0.05). The relative expression levels of PERK and ATF4 protein in the mimics group were (0.53±0.07) and (0.75±0.06). Compared with the mimics group, the relative expression levels of PERK and ATF4 proteins in the NG group and the blank group were both decreased (P<0.05). The results of MTT analysis showed that there was a statistically significant difference in the proliferation ability of the three groups of cells (F=21.44,28.38,59.14,P<0.05).The proliferation ability of Hep-2 cells in the mimics group was (0.15±0.01), (0.33±0.03) and (0.47±0.04) at 12, 24, and 36 h, respectively. Compared with the mimics group, the prolifera-tion ability of Hep-2 cells in the NG group and the blank group was significantly increased after 12, 24, and 36 h (P<0.05). The differ-ence in apoptosis rate of the three groups of transfected cells was statistically significant (F=102.32,P<0.05).Among them, the apoptosis rate of Hep-2 cells in the mimics group was (10.18±0.93). Compared with the mimics group, the apoptosis rate of the NG group and the blank group were both decreased (P<0.05).Conclusions Up-regulating the expression of miR-107 can inhibit the proliferation of Hep-2 cells and promote the apoptosis of Hep-2 cells. Endoplasmic reticulum stress may play an important role in it. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
