| 吴月霞,蒙毅,龚纯,等.运用网络药理学方法探讨安胃二号方治疗胃癌前病变的作用机制[J].安徽医药,2022,26(7):1292-1296. |
| 运用网络药理学方法探讨安胃二号方治疗胃癌前病变的作用机制 |
| Elucidation of the mechanism of Anwei prescription-2 in the treatment of gastric precancerous lesions based on network pharmacology |
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| DOI：10.3969/j.issn.1009-6469.2022.07.005 |
| 中文关键词: 胃肠疾病 癌前状态 网络 Meta分析 安胃二号方 网络药理学 前列腺素内过氧化物合酶 2 白细胞介素 6 丝裂原活化蛋白激酶 3 基质金属蛋白酶 3 炎性趋化因子 2 |
| 英文关键词: Gastrointestinaldisease Precancerousstate Networkmeta-analysis Anwei prescription-2 Network pharmacology Prostaglandin endoperoxide synthase 2 Interleukin 6 Mitogen-activated protein kinase 3 Matrix metalloproteinase 3 Inflam-matory chemokine 2 |
| 基金项目:广西自然科学基金项目( 2018GXNSFAA281063) |
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| 中文摘要: |
| 目的基于网络药理学方法探讨安胃二号方(全国名老中医林寿宁教授的经验方)治疗胃癌前病变的作用机制。方法本研究起止时间为 2020年 2—3月。通过中药系统药理学数据库和分析平台收集安胃二号方的有效化合物及靶点，经 GEO数据库筛选胃癌前病变的差异表达基因；利用 Venny软件获得安胃二号方治疗胃癌前病变的作用靶点，通过 Sytoscape软件创建安胃二号方治疗胃癌前病变的作用网络，在 String数据库完成蛋白互作网络；运用 DAVID数据库及京都基因与基因组百科全书对安胃二号方治疗胃癌前病变的作用靶点进行 GO分析和 KEGG分析。结果筛选得到安胃二号方的有效化合物 57个、有效靶点 233个，其中有 23个化合物及 31个靶点作用于胃癌前病变；蛋白互作网络中有 14个蛋白的作用度值在平均节点度以上， GO分析得到 342个生物过程和 19个细胞成分， KEGG分析得到 4条与胃癌前病变相关的通路。结论安胃二号方治疗胃癌前病变可能通过影响前列腺素内过氧化物合酶 2(PTGS2)、白细胞介素( IL)-6、丝裂原活化蛋白激酶 3(MAPK3)、基质金属蛋白酶 3(MMP3)、炎性趋化因子 2(CXCL2)等基因的表达水平，参与对 IL-17信号通路、 HIF-1信号通路、 TNF信号途径、 VEGF信号通路的调控，通过改变细胞结构及相关生物过程，减轻胃黏膜炎症损伤，促进细胞形态及功能恢复，抑制细胞增殖侵袭，阻止甚至逆转胃黏膜慢性炎症向胃癌前病变发展，达到治疗胃癌前病变的目的。 |
| 英文摘要: |
| Objective To explore the mechanism of Anwei prescription-2 for the treatment of gastric precancerous lesions based on network pharmacology.Methods This study period was from February to March 2020. The effective compounds and targets of Anwei prescription-2 were collected through the traditional Chinese medicine system pharmacology database and analysis platform, and thedifferentially expressed genes of gastric precancerous lesions were screened by the GEO database. The targets of Anwei prescription-2 in the treatment of gastric precancerous lesions were obtained through Venny software. The Anwei Prescription-2 network in the treat-ment of gastric precancerous lesions was established by Sytoscape software, and the protein interaction network was completed inSTRING database. DAVID database, GO and KEGG analyses were carried out on the targets of Anwei prescription-2 in the treatment of gastric precancerous lesions. Results There were 57 effective compounds and 233 effective targets of Anwei prescription-2 were screened, of which 23 compounds and 31 targets act on gastric precancerous lesions. There were 14 proteins in the protein interactionnetwork whose degree of action was above the average node degree. GO analysis obtained 342 biological processes and 19 cellular com-ponents, and KEGG analysis obtained 4 pathways related to gastric precancerous lesions.Conclusions Anwei prescription-2 may af-fect the expression levels of genes such as prostaglandin endoperoxide synthase 2 (PTGS2), interleukin 6 (IL6), mitogen-activated pro-tein kinase 3 (MAPK3), matrix metalloproteinase 3 (MMP3), and inflammatory chemokine 2 (CXCL2) to regulate the IL-17 signaling pathway, HIF-1 signaling pathway, TNF signaling pathway and VEGF signaling pathway, which will change the cell structure and relat-ed biological processes, reduce the inflammatory damage of gastric mucosa, promote the recovery of cell morphology and function, in-hibit cell proliferation and invasion, and prevent or even reverse the development of gastric mucosal chronic inflammation to precancer-ous lesions to treat gastric precancerous lesions. |
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