| 柳冬梅,赵慧.高效液相色谱 -电雾式检测器法测定尿素乳膏含量[J].安徽医药,2022,26(10):1929-1932. |
| 高效液相色谱 -电雾式检测器法测定尿素乳膏含量 |
| Determination of the content of urea cream by HPLC-CAD |
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| DOI：10.3969/j.issn.1009-6469.2022.10.005 |
| 中文关键词: 尿素 软膏 高效液相色谱 -电雾式检测器 含量测定 质量控制 高，分， |
| 英文关键词: Urea Ointments High performance liquid chromatography-charged aerosol detector Content determination Quality control |
| 基金项目:江苏省泰州市科技支撑计划(社会发展)项目指令性计划( TS201910) |
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| 中文摘要: |
| 目的建立 HPLC-CAD(高效液相色谱 -电雾式检测器)法测定尿素乳膏。方法本研究起止时间为 2019年 5月至 2020年 3月，利用 Waters XBridge Amide(4.6 mm×250 mm，3.5 μm)酰胺色谱柱；水-乙腈(10∶90)为流动相；流速为 1.0 mL/min；柱温为 35 ℃；CAD的参数设置:雾化温度为 35 ℃；幂率为 0.9；采集频率为 10 Hz；过滤常数为 10 s。结果尿素在 0.005～0.500 g/L的浓度范围内呈良好的线性关系( r=0.999)定量限(S/N=10)为 5 ng，检测限(S/N=3)为 2.5 ng，回收率为 100.2%，相对标准差( RSD)= |
| 英文摘要: |
| Objective To establish an HPLC-CAD (high performance liquid chromatography-charged aerosol detector) method for the determination of the content of urea cream.Methods This research was conducted from May 2019 to March 2020. The chromato.graphic column was Waters XBridge Amide (4.6 mm×250 mm, 3.5 μm). The mobile phase was composed of acetonitrile-water (90∶ 10). The flow rate was set at 1.0 mL/minand the column temperature was maintained at 35 ℃. The detector of CAD was applied withnebulization temperature at 35 ℃, and collection frequency at 10 Hz, and filter constant at 10 s, and power function value (PFV) at 0.9.Results The linearity of urea was fine in the range of 0.005～0.5 g/L with the correlation coefficient of 0.999. The limit of quantita.tion (S/N=10) was 5 ng and the limit of detection (S/N=3) was 2.5 ng. And the average recovery was 100.2%, with RSD being 0.71%.Conclusion The new established HPLC-CAD method shows better sensitivity, convenience, and durability for the determination ofthe content in urea cream, and is suitable for batch analysis, which could be a new method for the quality control of urea cream. |
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