| 张宪.高效液相色谱法检测七味都气丸中活性成分五味子醇甲、马钱苷和 23-乙酰泽泻醇 B的含量[J].安徽医药,2022,26(10):1933-1936. |
| 高效液相色谱法检测七味都气丸中活性成分五味子醇甲、马钱苷和 23-乙酰泽泻醇 B的含量 |
| Determination of contents of active ingredients of schisandrin, loganin and 23-acetyl alisol B in Qiweiduqi pills by HPLC |
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| DOI：10.3969/j.issn.1009-6469.2022.10.006 |
| 中文关键词: 主成分分析 色谱法，高压液相 七味都气丸 五味子醇甲 马钱苷 23-乙酰泽泻醇 B |
| 英文关键词: Principal component analysis Chromatography,high pressure liquid Qiweiduqi pills Schisandrin Loganin 23-acetyl alisol B |
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| 中文摘要: |
| 目的研究建立采用高效液相色谱法检测七味都气丸中活性成分五味子醇甲、马钱苷和 23-乙酰泽泻醇 B含量的分析方法。方法研究起止时间为 2019年 3月至 2022年 5月。七味都气丸样品经粉碎， 90%甲醇水溶液超声提取后，采用 Waters X-bridge C18色谱柱分离，柱温 27 ℃，检测波长 228 nm，进样体积 25 μL，流动包括流动相 A(乙腈)，流动相 B(0.3%磷酸水溶液)，梯度洗脱，洗脱程序为 0~7 min 8%A，7~18 min 8%~60%A，18~28 min 60%~91%A，28~30 min 91%~8%A，30~35min 8%A，外标法定量检测。结果马钱苷、五味子醇甲和 23-乙酰泽泻醇 B在质量浓度 0.1~20.0 mg/L范围内具有良好的线性关系，相关系数( r)均大于 0.995；重复性 RSD(n=6)分别为 2.7%、3.1%和 3.4%；加样回收率分别为 99.67%、100.11%和 100.49%。结论该方法具有简便、快速、准确度好等优点，适用于七味都气丸品种的质量控制。 |
| 英文摘要: |
| Objective To develop an analytical method for determinating contents of active ingredients of schisandrin, loganin and 23-acetyl alisol B in Qiweiduqi pills by high performance liquidchromatography (HPLC).Method The study starts and ends from March 2019 to may 2022. After Qiweiduqi pill samples were crushed and ultrasonic extracted with 90% methanol solution, Waters X-bridge C18 column was selected for separation, with column temperature of 27℃. Detection wavelength was set at 228 nm, injection vol.ume was 25 μL. Mobile phase, acetonitrile (A) and 0.3% phosphoric acid solution( B). Gradient elution procedure was as follows, 0-7 min 8%A,7-18 min 8%-60%A,18-28 min 60%-91%A,28-30 min 91%-8%A,30-35min 8%A. Quantitative analysis was performed by external standard.Result Schisandrin, loganin and 23-acetyl alisol B showed good linear relationships in the concentration range of |
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