| 张晶,安秀英,胡锋超.微小核糖核酸 -200b-3p、血管内皮生长因子 A在食管鳞状细胞癌组织中的表达及其与复发转移相关性[J].安徽医药,2022,26(10):1954-1959. |
| 微小核糖核酸 -200b-3p、血管内皮生长因子 A在食管鳞状细胞癌组织中的表达及其与复发转移相关性 |
| Expression of microRNA-200b-3p and vascular endothelial growth factor A in esophageal squamous cell carcinoma tissue and their correlation with recurrence and metastasis |
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| DOI：10.3969/j.issn.1009-6469.2022.10.011 |
| 中文关键词: 食管肿瘤 癌，鳞状细胞 血管内皮生长因子 微小核糖核酸 -200b-3p 复发转移 相关性 |
| 英文关键词: Esophageal neoplasms Carcinoma,squamous cell Vascular endothelial growth factor microRNA-200b-3p Re. currence and metastasis Correlation |
| 基金项目:邢台市科技计划项目( 2018ZC127) |
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| 中文摘要: |
| 目的探讨食管鳞状细胞癌组织中微小核糖核酸 -200b-3p(miR-200b-3p)、血管内皮生长因子 A(VEGFA)表达及其与复发转移的关系。方法回顾性收集 2013年 12月至 2015年 12月于河北省退役军人总医院经手术切除且经组织病理证实的 95例食管鳞状细胞癌病人的食管鳞状细胞癌组织及相应的癌旁正常组织。采用实时荧光定量 PCR法检测组织中 miR-200b-3p、VEGFA mRNA表达，采用免疫组化法检测组织中 VEGFA表达。采用 Pearson检验分析食管鳞状细胞癌病人 miR-200b-3p与 VEGFA mRNA表达的相关关系；随访 5年，采用 Kaplan-Meier法分析食管鳞状细胞癌组织中 miR-200b-3p、VEGFA表达与病人复发转移的关系，采用 Cox比例风险模型分析影响食管鳞状细胞癌病人复发转移的危险因素。结果与癌旁正常组织比较，食管鳞状细胞癌组织中 miR-200b-3p表达水平明显降低［( 1.00±0.24)比( 0.67±0.18)P<0.05］VEGFA mRNA和 VEGFA阳性率明显升高［( 1.01±0.26)比( 1.92±0.50)、(17.89%)比( 78.95%)， P<0.05］。食管鳞状细，胞癌病，人 miR-200b-3p与 VEGFA mRNA表达呈负相关( r=.0.39，P<0.05)。与非复发转移组比较，复发转移组病人食管鳞状细胞癌组织中 miR-200b-3p表达水平明显降低［(0.78±0.20)比( 0.53±0.14)P<0.05］VEGFA阳性率明显升高［(67.92%)比( 92.86%)，P<0.05］。食管鳞状细胞癌组织中 miR-200b-3p、VEGFA表达与病人，组织分化，程度、 TNM分期有关( P<0.05)。 miR-200b-3p低表达者 5年复发转移率 |
| 英文摘要: |
| Objective To investigate the expression of microRNA-200b-3p (miR-200b-3p) and vascular endothelial growth factor A(VEGFA) in esophageal squamous cell carcinoma tissue and their relationship with recurrence and metastasis.Methods Esophagealsquamous cell carcinoma tissue and corresponding adjacent normal tissue surgically resected and confirmed by histopathology of 95 pa.tients, who were admitted to Hebei General Hospital for Veterans from December 2013 to December 2015, were collected retrospective.ly. The expressions of miR-200b-3p and VEGFA mRNA were detected by real-time fluorescence quantitative PCR, and the expressionof VEGFA was detected by immunohistochemistry. Pearson test was used to analyze the correlation between miR-200b-3p and VEGFA mRNA expressions in patients with esophageal squamous cell carcinoma. After 5 years of follow-up, Kaplan-Meier method was used to analyze the relationship between the expressions of miR-200b-3p and VEGFA and the recurrence and metastasis of esophageal squa.mous cell carcinoma; Cox proportional risk model was used to analyze the risk factors of recurrence and metastasis in patients withesophageal squamous cell carcinoma.Results Compared with normal adjacent tissues, miR-200b-3p expression level in esophageal squamous cell carcinoma tissues was significantly decreased [(1.00±0.24) vs. (0.67±0.18); P<0.05], VEGFA mRNA and VEGFA posi. tive rates were significantly increased [(1.01±0.26) vs. (1.92±0.50), 17.89% vs. 78.95%; P<0.05]. There was a negative correlation be. tween miR-200b-3p and VEGFA mRNA expressions in patients of esophageal squamous cell carcinoma (r=.0.39, P<0.05). Compared with non-recurrent metastasis group, the expression level of miR-200b-3p in recurrent metastasis group was significantly decreased [(0.78±0.20) vs. (0.53±0.14); P<0.05] , and the positive rate of VEGFA was significantly increased (67.92% vs. 92.86%;P<0.05). The expressions of miR-200b-3p and VEGFA in esophageal squamous cell carcinoma tissue was related to the degree of tissue differentia. tion and TNM staging (P<0.05). The 5-year recurrence and metastasis rateof patients with low expression of miR-200b-3p was signifi. cantly higher than that of patients with high expression(55.56% vs. 34.00%, P<0.05); the 5-year recurrence and metastasis rate of highexpression of VEGFA was significantly higher than that of patients with low expression(55.17% vs. 27.03%, P<0.05). Tissue differentia. tion, low expression of miR-200b-3p and high expression of VEGFA were independent risk factors for recurrence and metastasis ofesophageal squamous cell carcinoma (P<0.05).Conclusion The expression of miR-200b-3p was down regulated and the expression of VEGFA was up-regulated in esophageal squamous cell carcinoma tissue, both of which can be used as clinical reference indexes toevaluate the recurrence and metastasis of the disease. |
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