| 郭春辉,梁科峰,张小伟.长链非编码 RNA LINC00662靶向微小 RNA-103a-3p对黑色素瘤细胞增殖和周期的影响[J].安徽医药,2022,26(10):2058-2062. |
| 长链非编码 RNA LINC00662靶向微小 RNA-103a-3p对黑色素瘤细胞增殖和周期的影响 |
| Effect of lncRNA LINC00662 targeting microRNA-103a-3p on the proliferation and cell cycle of cutaneous melanoma cells |
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| DOI：10.3969/j.issn.1009-6469.2022.10.035 |
| 中文关键词: 黑色素瘤 长链非编码 RNA LINC00662 微小 RNA-103a-3p 细胞增殖 细胞周期蛋白 D1 增殖细胞核抗原 |
| 英文关键词: Melanoma LINC00662 miR-103a-3p Cell proliferation Cyclin D1 Proliferating cell nuclear antigen |
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| 中文摘要: |
| 目的探究长链非编码 RNA LINC00662对黑色素瘤细胞增殖和周期的影响，以及分子机制。方法本研究于 2018年 10月至 2020年 2月进行。以体外培养的人皮肤黑色素瘤细胞 A375为研究对象，然后将其分为四组，分别为 si-NC、si-LINC00662、si-LINC00662+anti-miR-NC和 si-LINC00662+anti-miR-103a-3p转染 A375细胞。采用 RT-PCR检测皮肤黑色素瘤组织中 LINC00662和 miR-103a-3p的表达水平， CCK-8法检测细胞增殖，流式细胞仪检测细胞周期，蛋白质印迹法( western blot. ting)检测细胞周期蛋白 1(CyclinD1)、增殖细胞核抗原( PCNA)的蛋白表达。结果与正常组织和正常皮肤细胞相比，在皮肤黑色素瘤组织和细胞株中 LINC00662表达［ 1.24±0.50比 4.17±1.27］上调， miR-103a-3p表达［ 1.17±0.32比 0.64±0.21］显著下调。 LINC00662与 miR-103a-3p靶向结合。与 si-NC组相比， si-LINC00662组 LINC00662表达［ 1.00±0.06比 0.48±0.06］、 OD值和 S期细胞比例［( 34.7±3.5)%比( 20.5±3.0)%］降低， G0/G1期细胞比例［( 57.4±5.2)%比( 74.7±4.8)%］增高， Cyclin D1［1.00±0.01比 |
| 英文摘要: |
| Objective To investigate the effect of LINC00662 on the proliferation and cell cycle of cutaneous melanoma cells and its potential mechanism.Methods The study was conducted from October 2018 to February 2020. Human skin cutaneous melanoma cell line A375 cultured in vitro was used as the research object, then it was divided into four groups, and cells were transfected with si-NC, si-LINC00662, si-LINC00662+anti-miR-NC, and si-LINC00662+anti-miR-103a-3p. The expression levels of LINC00662 and miR-103a-3p were detected by RT-PCR, cell proliferation was detected by CCK-8 method, cell cycle was detected by flow cytometry, and the expression of CyclinD1 and PCNA were detected by Western blotting.Results Compared with normal tissues and normal skin cells, the expression of LINC00662 [(1.24±0.50) vs. (4.17±1.27)] was significantly up-regulated and the expression of miR-103a-3p [(1.17±0.32) vs. (0.64±0.21)] was significantly down-regulated in skin cutaneous melanoma tissues and cell lines. LINC00662 binded to miR-103a-3p. Compared with si-NC group, the expression of LINC00662 [(1.00±0.06) vs. (0.48±0.06)] , OD value and the proportion of cells in S phase [(34.7±3.5) % vs. (20.5±3.0) %] were decreased in si-LINC00662 group, the proportion of cells in G0/G1 phase [(57.4± 5.2) % vs. (74.7±4.8) %] was increased, and the protein expression of Cyclin D1 [(1.00±0.01) vs. (0.56±0.06)] and PCNA [(0.99±0.05) vs. (0.53±0.06)] was decreased. Inhibition of miR-103a-3p expression partially restored the effect of LINC00662 on the proliferation and cell cycle of melanoma cells.Conclusion LINC00662 negatively regulates the expression of miR-103a-3p, and inhibits skin cuta. neous melanoma cell proliferation and induces cell cycle arrest. |
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