| 杨双,俞思全.紫草素促进食管癌细胞凋亡及细胞周期阻滞的作用机制研究[J].安徽医药,2023,27(1):79-82. |
| 紫草素促进食管癌细胞凋亡及细胞周期阻滞的作用机制研究 |
| Effect and mechanism of shikonin on apoptosis and cell cycle arrest of esophageal cancer cells |
| |
| DOI：10.3969/j.issn.1009-6469.2023.01.017 |
| 中文关键词: 食管肿瘤 紫草素 凋亡 细胞周期 PI3K/AKT通路 |
| 英文关键词: Esophageal neoplasms Shikonin Apoptosis Cell cycle PI3K/Akt pathway |
| 基金项目: |
|
| 摘要点击次数: 957 |
| 全文下载次数: 241 |
| 中文摘要: |
| 目的研究紫草素促进食管癌细胞凋亡及细胞周期阻滞的作用及机制。方法 2019年 1月至 2020年 6月期间开展细胞实验，培养食管癌 Eca109细胞，分为不含药物杜氏改良 Eagle培养基( DMEM)处理的对照组，含不同剂量紫草素 DMEM处理的紫草素组，含 2.0 μmol/L紫草素和 10 μg/L胰岛素样生长因子 -1(IGF-1)DMEM处理的 2.0 μmol/L紫草素 +10μg/L IGF-1组。检测细胞凋亡率、细胞周期及凋亡基因活化的胱天蛋白酶 -3(cleaved caspase-3)、细胞周期蛋白 D1(cyclin D1)、磷酸化磷酸肌醇 3激酶( PI3K)、蛋白激酶 B(AKT)的表达量。结果 0.5 μmol/L紫草素组、 1.0 μmol/L紫草素组、 2.0 μmol/L紫草素组的细胞凋亡率、细胞周期 G1期比例、 cleaved caspase-3的表达量［ 0.5 μmol/L紫草素组( 0.64±0.14)、 1.0 μmol/L紫草素组( 0.81±0.19)、2.0 μmol/L紫草素组( 1.02±0.24)］高于对照组 0.41±0.07，细胞周期 S期、 G2期比例及 cyclin D1［0.5 μmol/L紫草素组( 0.80±0.16)、 1.0 μmol/L紫草素组( 0.68±0.13)、 2.0 μmol/L紫草素组( 0.45±0.09)］、 p-PI3K、p-AKT表达量低于对照组［ cyclin D1表达量(0.91±0.18)］(P<0.05)； 2.0 μmol/L紫草素 +10 μg/L IGF-1组的细胞凋亡率、细胞周期 G1期比例、 cleaved caspase-3的表达量低于 2.0 μmol/L紫草素组，细胞周期 G2期比例及 cyclin D1、p-PI3K、p-AKT表达量高于 2.0 μmol/L紫草素组( P<0.05)。结论紫草素具有促进食管癌细胞凋亡及细胞周期阻滞的作用，该作用与抑制 PI3K/AKT通路激活有关。 |
| 英文摘要: |
| Objective To study the effect and mechanism of shikonin on apoptosis and cell cycle arrest of esophageal cancer cells. Methods Cell experiments were carried out from January 2019 to June 2020. Esophageal cancer ECA 109 cells were cultured and assigned into groups: control group treated with Duchenne modified Eagle medium (DMEM) without drug, shikonin groups treated withDMEM containing different doses of shikonin, which included 2.0 μmol/L shikonin+10 μg/L insulin-like growth factor-1 (IGF-1) group treated with DMEM containing 2.0 μmol/L shikonin and 10 μg/L IGF-1. Apoptosis rate, cell cycle and the expressions of apoptotic gene-activated caspase-3 (cleaved caspase-3), cyclin D1, phosphorylated phosphoinositol 3 kinase (PI3K) and protein kinase B (AKT) were detected.Results The apoptotic rate, the proportion of G1 phase and the expression of cleaved caspase-3 [0.5 μmol/L shikoningroup (0.64±0.14), 1.0 μmol/L shikonin group (0.81±0.19), 2.0 μmol/L shikonin group (1.02±0.24)] in 0.5 μmol/L, 1.0 μmol/L and 2.0μmol/L shikonin groups were higher than those in the control group 0.41±0.07, while the proportions of S phase and G2 phase and theexpressions of cyclin D1 [0.5 μmol/L shikonin group (0.80±0.16), 1.0 μmol/L shikonin group (0.68±0.13), 2.0 μmol/L shikonin group(0.45±0.09)], p-PI3K and p-AKT were lower than those in the control group [cyclin D1 expression: (0.91±0.18)] (P<0.05). The apoptotic rate, the proportion of G1 phase and the expression of cleaved caspase-3 in 2.0 μmol/L shikonin+10 μg/L IGF-1 group were lower than those in 2.0 μmol/L shikonin group, while the proportion of G2 phase and the expressions of cyclin D1, p-PI3K and p-AKT were higher than those in 2.0 μmol/L shikonin group (P<0.05).Conclusion Shikonin can promote the apoptosis and cell cycle arrest of esophagealcancer cells, which is related to the inhibition of PI3K/Akt pathway activation. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
