| 张子杭,成元华,李小梅.转化生长因子-β 通路对胃癌细胞增殖、侵袭及slug 蛋白、上皮钙黏素表达的影响[J].安徽医药,2023,27(3):486-490. |
| 转化生长因子-β 通路对胃癌细胞增殖、侵袭及slug 蛋白、上皮钙黏素表达的影响 |
| Effects of TGF-β pathway on proliferation, invasion and expression of slug and E-cad proteins in gastric cancer HGC-27 cells |
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| DOI：10.3969/j.issn.1009-6469.2023.03.014 |
| 中文关键词: 胃肿瘤 转化生长因子β(TGF-β) Snail家族转录因子类 钙黏着糖蛋白类 信号通路 |
| 英文关键词: Stomach neoplasms Transforming growth factor beta(TGF-β) Snail family transcription factors Cadherins Sig?nal pathway |
| 基金项目:贵州省科学技术基金项目(黔科合J字［2009］2187号) |
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| 中文摘要: |
| 目的 研究转化生长因子-β(TGF-β)通路的激活与阻断对人胃癌HGC-27细胞增殖、侵袭及slug蛋白和上皮钙黏素(E-cadherin，E-cad)表达的影响。方法 于2021年1月至2022年1月使用外源性通路激活剂TGF-β1和阻断剂SB431542培养胃癌细胞，后根据对细胞给药不同分为五组。空白对照组:仅使用等量常规培养基；激动剂组:TGF-β1(25 μg/L)；阻断剂组:SB431542(2 mg/L)；激动剂+低浓度阻断剂组:TGF-β1(25 μg/L)+SB431542(2 mg/L)；激动剂+高浓度阻断剂组:TGF-β1(25 μg/L)+SB431542(4 mg/L)。并使用细胞计数试剂盒(CCK-8)法检测各组胃癌细胞增殖活性，使用Transwell侵袭实验检测各组胃癌细胞侵袭能力，使用酶联免疫吸附测定(ELISA)检测胃癌细胞中slug与E-cad两种蛋白的表达情况。结果 五组胃癌细胞增殖活性、侵袭能力及slug蛋白、E-cad蛋白表达水平比较均差异有统计学意义(P<0.05)。激动剂组胃癌细胞增殖活性(24 h:0.70±0.13)和侵袭能力［细胞穿过小室膜的细胞数量(227.45±5.46)个］、slug蛋白表达水平［(149.45±24.46)ng/L］高于空白对照组［24 h:0.52±0.10、(117.81±3.11)个、(101.25±20.11)ng/L］(P<0.05)，而E-cad蛋白表达水平(23.54±9.42)ng/L相较空白对照组(41.56±25.26)ng/L显著降低(P<0.05)。结论 TGF-β通路可通过增强slug蛋白表达并抑制E-cad表达，激活上皮-间充质转化(EMT)，提高胃癌细胞的体内外增殖和侵袭能力，从而影响胃癌的进展。 |
| 英文摘要: |
| Objective To explore the influence of transforming growth factor- β (TGF- β) pathway activation and disruption on the proliferation and invasion of human gastric cancer HGC-27 cells and the expressions of slug and E-cadherin (E-cad).Methods From January 2021 to January 2022, gastric cancer cells cultured with exogenous pathway activator (TGF-β1) and blocker (SB431542) were assigned into 5 groups according to the different drug administration for the cells: blank control group: using only the same amount of conventional medium, agonist group: TGF-β1 (25 μg/L), blocker group: SB431542 (2 mg/L), agonist + low-concentration blocker group:TGF-β1 (25 μg/L) +SB431542 (2 mg/L), and agonist + high-concentration blocker group: TGF-β1 (25 μg/L) +SB431542 (4 mg/L). The Cell Counting Kit-8 (CCK-8) was used to detect the proliferation activity of gastric cancer cells in each group, Transwell was used to de?tect the invasion ability of gastric cancer cells in each group, and the expressions of slug and E-cad in gastric cancer cells was detected by ELISA.Results There were significant differences in the proliferation activity, invasion ability and the expression levels of slug protein and E-cad protein among the five groups (P<0.05). Proliferative activity of gastric cancer cells (24 h: 0.70±0.13), invasion abili?ty [the number of cells passing through the capsule membrane (227.45±5.46)], and expression level of slug protein [(149.45±24.46) ng/L] in agonist group were higher than those of the blank control group [24 h: 0.52±0.10, 117.81±3.11, (101.25±20.11) ng/L] (P<0.05),but the expression level of E-cad protein was significantly lower in agonist group than in control group [(23.54±9.42) ng/L vs. (41.56±25.26) ng/L; P<0.05].Conclusion TGF-β pathway can enhance the proliferation and invasion ability of gastric cancer cells in vitro and in vivo by enhancing the expression of slug protein and inhibiting the expression of E-cad, activating epithelial-mesenchymal tran?sition (EMT), thus affecting the progression of gastric cancer. |
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