| 陈晶晶,陈 炯.柠檬酸和EDTA对cyclinD1和TdT抗原修复的应用比较[J].安徽医药,2015,19(10):1901-1904. |
| 柠檬酸和EDTA对cyclinD1和TdT抗原修复的应用比较 |
| Comparison of citrate with EDTA buffer in antigen retrieval of cyclinD1 and TdT |
| 投稿时间：2015-04-10 |
| DOI： |
| 中文关键词: 抗原修复 套细胞淋巴瘤 淋巴母细胞淋巴瘤 cyclinD1 TdT |
| 英文关键词: antigen retrieval mantle cell lymphoma lymphoblastic lymphomas cyclinD1 TdT |
| 基金项目:安徽省2013年第三批科研计划项目(No 1301043013) |
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| 中文摘要: |
| 目的 探讨pH 6.0 Citrate、pH 8.0 Tris/EDTA和pH 9.0 Tris/EDTA三种修复液对恶性淋巴瘤中cyclinD1和TdT抗原修复的比较。方法 运用高压抗原修复法,选择三种修复液,比较56例套细胞淋巴瘤cyclinD1和48例淋巴母细胞型淋巴瘤TdT的免疫组化染色效果的比较。结果 免疫组化染色采用柠檬酸和EDTA两种修复液相比,56例套细胞淋巴瘤中40例cyclinD1表达强度随着pH值的升高而增强,6例用pH 6.0 Citrate修复后不表达,而用pH 9.0 Tris/EDTA修复后表达；另外48例淋巴母细胞型淋巴瘤中42例TdT表达强度随着pH值的升高而增强,其中4例用pH 6.0 Citrate修复后不表达,而用高pH值EDTA修复后表达。结论 对于这两种抗体cyclinD1和TdT来说,以高压热修复,高pH值EDTA抗原修复溶液优于pH 6.0 Citrate,提高了恶性淋巴瘤中cyclinD1和TdT抗原表达。 |
| 英文摘要: |
| Objective To compare citrate with EDTA buffers in antigen retrieval for cyclinD1 and TdT staining in malignant lymphoma.Methods Using pressure cooker heat-induced antigen retrieval,citrate and EDTA were compared in cyclinD1 immunostaining in 56 cases of mantle cell lymphoma and TdT immunostaining in 48 cases of lymphoblastic lymphomas.Results Positive expression rate of cyclinD1 was enhanced in 40 of 56 cases and positive expression rate of TdT in 42 of 48 cases were enhanced as pH of retrieval buffer was enhanced.Six cases MBL and four cases LBL were negative by citrate retrieval but were strong by EDTA buffer.Conclusions The use of pressure cooker heat-induced antigen retrieval and EDTA buffers which were compared with citrate buffer could enhance the immunohischemical staining of cyclinD1 and TdT in malignant lymphoma. |
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