| 史冠男,袁媛,彭琼,等.CCL21对胃癌SGC-7901细胞发生上皮间质转化的影响[J].安徽医药,2017,21(1):113-115. |
| CCL21对胃癌SGC-7901细胞发生上皮间质转化的影响 |
| Effects of CCL21 on inducing epithelial-mesenchymal transition in gastric cancer cells SGC-7901 |
| 投稿时间：2016-08-10 |
| DOI： |
| 中文关键词: 上皮间质转化 趋化因子 胃癌 |
| 英文关键词: Epithelial-mesenchymal transition Chemokine Gastric cancer |
| 基金项目:安徽省合肥市重点科技项目(JZ201512250023) |
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| 中文摘要: |
| 目的 研究CCL21能否参与诱导胃癌SGC-7901细胞发生上皮间质转化。方法 取不同浓度(0、50、100、150 μg·L-1)的CCL21作用胃癌SGC-7901细胞,划痕试验检测该细胞侵袭能力。镜下观察该细胞形态的变化。Western blot分别检测E-cadherin、N-cadherin、Vimentin、MMP-9的蛋白表达。结果 150 μg·L-1 浓度的CCL21作用胃癌SGC-7901细胞后,较其它浓度侵袭能力强,同时使胃癌SGC-7901细胞的胞体逐渐变为长梭状,部分可见伪足生成。E-cadherin蛋白表达下降(P<0.05),N-cadherin、Vimentin、MMP-9蛋白表达升高(P<0.05)。结论 CCL21可能参与诱导胃癌SGC-7901细胞发生上皮间质转化。 |
| 英文摘要: |
| Objective To investigate whether CCL21 can induce epithelial-mesenchymal transition in gastric cancer cells SGC-7901. Methods SGC-7901 cells were induced by CCL21 in different concentration (0,0,100,0 μg·L-1) and then the ability of invasion and metastasis was detected by scratch testing.Morphological changes in forms were observed.The protein expressions of E-cadherin,N-cadherin,Vimentin,MMP-9 were detected by Western blot. Results After being induced by CCL21 in 150 μg·L-1,SGC-7901 cells achieved faster ability of invasion and metastasis.SGC-7901 cells formed into a long spindle and pseudopod could be seen.The expressions of N-cadherin,Vimentin,and MMP-9 rose(P<0.05)while that of E-cadherin obviously descended(P<0.05). Conclusions CCL21 may induce epithelial-mesenchymal transition in gastric cancer cell and promote the invasion and metastasis. |
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